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Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics

Title
Phenotyping of Tianma-Stimulated Differentiated Rat Neuronal B104 Cells by Quantitative Proteomics
Author
Klaus Heese
Keywords
iTRAQ technology; Neural cell adhesion molecule 1; Neurodegenerative diseases; Tianma
Issue Date
2012-04
Publisher
KARGER, ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND
Citation
NEUROSIGNALS, 20, 1, 48-60
Abstract
Gastrodia elata blume (tianma) is a traditional Chinese herb often used in the treatment of convulsions, headaches, and hypertension. Although interest in neuronal- related actions of tianma is increasing, minimal studies have been conducted to determine its specific effects on neuronal cells. This study was designed to examine the effects of tianma on the metabolism in differentiated neuroblastoma cells using the isobaric tag for relative and absolute quantitation (iTRAQ) technology. Stimulation of these cells with tianma caused changes in the expression of 38 proteins that were subsequently classified according to their physiological functions and association with neurodegenerative diseases. We identified six proteins with altered functional activities in neurodegenerative disease states that were modulated by tianma: triosephosphate isomerase (Tpi1), peptidyl- prolyl cis- trans isomerase A (Ppia), neural cell adhesion molecule 1 (Ncam1), ubiquitin carboxyl- terminal hydrolase isozyme L1 (Uchl1), septin- 2 (Sept2) and heat shock protein 90 (Hsp90aa1). Wepostulate that tianma mediates its neuroprotective effects via upregulation of Ncam1, Hsp90aa1, Tpi1 and Ppia while downregulating Sept2 and Uchl1. These changes in protein expression aid in the restoration of the intracellular environment to a metabolically balanced state, promoting cell survival. Based on these observed data, we conclude that tianma has therapeutic potential, especially for neurodegenerative diseases. Copyright (C) 2011 S. Karger AG, Basel
URI
https://www.karger.com/Article/Abstract/331492http://hdl.handle.net/20.500.11754/37860
ISSN
1424-862X
DOI
10.1159/000331492
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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