Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임태연 | - |
dc.date.accessioned | 2018-02-15T20:18:55Z | - |
dc.date.available | 2018-02-15T20:18:55Z | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | American Journal of Respiratory & Critical Care Medicine (AM J RESPIR CRIT CARE MED), Vol.184, No.5 [2011], p528-536 | en_US |
dc.identifier.issn | 1073-449X | - |
dc.identifier.uri | https://www.atsjournals.org/doi/abs/10.1164/rccm.201006-0951OC | - |
dc.description.abstract | RATIONALE: Airway inflammation and remodeling during asthma are attributed to the altered expression of biologically relevant proteins. OBJECTIVES: To search for asthma-specific proteins in bronchoalveolar lavage fluid (BAL) from individuals with asthma and to validate the identified proteins in an experimental model of asthma. METHODS: Liquid chromatography-tandem mass spectrometry was performed to identify proteins in BAL fluid found by two dimensional electrophoresis (2DE) to be differentially expressed in subjects with asthma versus control subjects. Group-specific component (Gc) and mRNA levels were measured using an ELISA, Western blots, and PCR. A neutralization study using an antibody against Gc protein was performed in an experimental asthma model. MEASUREMENTS AND MAIN RESULTS: Based on 2DE, 15 proteins were significantly up-regulated or down-regulated in eight subjects with asthma compared with eight control subjects. The protein levels of Gc, hemopexin, and haptoglobin-b were increased, whereas the a1- antitrypsin and glutathione S-transferase levels were decreased in subjects with asthma. The Gc concentration in BAL fluid was significantly elevated in 67 subjects with asthma compared with that in 22 control subjects (P < 0.009). The Gc was significantly correlated with the neutrophil percentage in BAL fluid of subjects with asthma (P = 0.001). Gc mRNA and protein levels were higher in ovalbumin-sensitized/ challenged asthma mice than in sham-treated mice. Gc protein were expressed on alveolar macrophages and on epithelial cells. Treatment with an anti-Gc antibody dose-dependently reduced the ovalbumin sensitization/challenge-induced enhancement of airway hyperreactivity, airway inflammation, goblet cell hyperplasia,and levels of eotaxin, interleukin-4, -5, and -13, and interferon-g. CONCLUSIONS: Gc may be involved in the development of asthma, and the neutralization of Gc protein could be a therapeutic strategy for asthma. | en_US |
dc.description.sponsorship | Supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare, and Family Affairs, Republic of Korea (A090548). | en_US |
dc.language.iso | en | en_US |
dc.publisher | MEDLINE | en_US |
dc.subject | 2D electrophoresis | en_US |
dc.subject | Asthma | en_US |
dc.subject | BAL | en_US |
dc.subject | Gc | en_US |
dc.subject | Proteomics | en_US |
dc.subject | Therapeutics | en_US |
dc.title | Relationship between Group-Specific Component Protein and the Development of Asthma | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 184 | - |
dc.identifier.doi | 10.1164/rccm.201006-0951OC | - |
dc.relation.page | 528-536 | - |
dc.relation.journal | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | - |
dc.contributor.googleauthor | Lee, Shin-Hwa | - |
dc.contributor.googleauthor | Kim, Kyung-Hun | - |
dc.contributor.googleauthor | Kim, Jin-Moo | - |
dc.contributor.googleauthor | Yoon, Sang-Hyuk | - |
dc.contributor.googleauthor | Kim, Tae-Hoon | - |
dc.contributor.googleauthor | Park, Sung-Woo | - |
dc.contributor.googleauthor | Park, Jong-Sook | - |
dc.contributor.googleauthor | Uh, Soo-Taek | - |
dc.contributor.googleauthor | Lee, Ho-Sung | - |
dc.contributor.googleauthor | Chung, Il-Yup | - |
dc.relation.code | 2011200530 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | rhim | - |
dc.identifier.researcherID | 15742259000 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.