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Log-scale dose response of inhibitors on a chip

Title
Log-scale dose response of inhibitors on a chip
Author
Jong Wook Hong
Keywords
MATRIX-METALLOPROTEINASE INHIBITORS; MICROFLUIDIC DEVICE; KINETIC-PARAMETERS; ENZYME ASSAYS; GENERATION; MICROCHIP; GRADIENT; READER; CELLS
Issue Date
2011-08
Publisher
AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Citation
ANALYTICAL CHEMISTRY, 권: 83, 호: 16, 페이지: 6148-6153
Abstract
We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirec 0000005E t dilution capability of reagents for the determination of the half inhibitory concentration o 00000AEC r IC(50). The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC(50) of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry.
URI
http://pubs.acs.org/toc/ancham/83/16/?http://hdl.handle.net/20.500.11754/37830
ISSN
0003-2700
DOI
10.1021/ac201177g
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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