Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-02-15T12:17:31Z | - |
dc.date.available | 2018-02-15T12:17:31Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Transplant International. Feb 01, 2012 25(2):242-249 | en_US |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2011.01394.x/full | - |
dc.description.abstract | This study investigated the effect of local glucagon-like peptide-1 (GLP-1) production within mouse islets on cytoprotection in vitro and in vivo by gene transfer of GLP-1. Transduction of recombinant adenovirus vector expressing GLP-1 (rAd-GLP-1) induced a significant increase in bioactive GLP-1 in the mouse islet culture, whereas transduction with adenovirus vector expressing β-galactosidase (rAd-LacZ), as a control, had no effect on GLP-1 secretion. Islets transduced with rAd-GLP-1 were protected from H2O2-induced cell damage in vitro. In addition, glucose-stimulated insulin secretion was significantly increased in rAd-GLP-1-transduced islets. When transplanted under the kidney capsule of diabetic syngeneic mice, islet grafts retrieved 4 or 7 days after transplantation revealed that the rAd-GLP-1-transduced group had significantly more Ki67-positive cells as compared with the rAd-LacZ-transduced group. Regarding blood glucose control, diabetic mice transplanted with a marginal mass of rAd-GLP-1-transduced islets became normoglycemic more rapidly and 78% of the recipients were normoglycemic at 35 days post-transplant, whereas only 48% of the mice transplanted with rAd-LacZ-transduced islets were normoglycemic (P < 0.05). In conclusion, delivery of the GLP-1 gene to islets enhanced islet cell survival during the early post-transplant period, and preserved islet mass and functions over time in the transplants. | en_US |
dc.description.sponsorship | This work was supported by a research grant from the Innovative Research Institute for Cell Therapy, Republic of Korea (A062260) to S.H.I. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | en_US |
dc.subject | glucagon-like peptide-1 (GLP-1) | en_US |
dc.subject | islet | en_US |
dc.subject | transplantation | en_US |
dc.title | Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 25 | - |
dc.identifier.doi | 10.1111/j.1432-2277.2011.01394.x | - |
dc.relation.page | 242-249 | - |
dc.relation.journal | TRANSPLANT INTERNATIONAL | - |
dc.contributor.googleauthor | Chae, Hee-Young | - |
dc.contributor.googleauthor | Kang, Jun-Goo | - |
dc.contributor.googleauthor | Kim, Chul-Sik | - |
dc.contributor.googleauthor | Lee, Seong-Jin | - |
dc.contributor.googleauthor | Lee, Min-hyung | - |
dc.contributor.googleauthor | Kang, Dong-chul | - |
dc.contributor.googleauthor | Jun, Hee-Sook | - |
dc.contributor.googleauthor | Ihm, Sung-Hee | - |
dc.relation.code | 2012212793 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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