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Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation

Title
Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation
Author
이민형
Keywords
glucagon-like peptide-1 (GLP-1); islet; transplantation
Issue Date
2012-02
Publisher
WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Citation
Transplant International. Feb 01, 2012 25(2):242-249
Abstract
This study investigated the effect of local glucagon-like peptide-1 (GLP-1) production within mouse islets on cytoprotection in vitro and in vivo by gene transfer of GLP-1. Transduction of recombinant adenovirus vector expressing GLP-1 (rAd-GLP-1) induced a significant increase in bioactive GLP-1 in the mouse islet culture, whereas transduction with adenovirus vector expressing β-galactosidase (rAd-LacZ), as a control, had no effect on GLP-1 secretion. Islets transduced with rAd-GLP-1 were protected from H2O2-induced cell damage in vitro. In addition, glucose-stimulated insulin secretion was significantly increased in rAd-GLP-1-transduced islets. When transplanted under the kidney capsule of diabetic syngeneic mice, islet grafts retrieved 4 or 7 days after transplantation revealed that the rAd-GLP-1-transduced group had significantly more Ki67-positive cells as compared with the rAd-LacZ-transduced group. Regarding blood glucose control, diabetic mice transplanted with a marginal mass of rAd-GLP-1-transduced islets became normoglycemic more rapidly and 78% of the recipients were normoglycemic at 35 days post-transplant, whereas only 48% of the mice transplanted with rAd-LacZ-transduced islets were normoglycemic (P < 0.05). In conclusion, delivery of the GLP-1 gene to islets enhanced islet cell survival during the early post-transplant period, and preserved islet mass and functions over time in the transplants.
URI
http://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2011.01394.x/full
ISSN
0934-0874
DOI
10.1111/j.1432-2277.2011.01394.x
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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