75 0

Fluoxetine Up-Regulates Bd-xL Expression in Rat C6 Glioma Cells

Title
Fluoxetine Up-Regulates Bd-xL Expression in Rat C6 Glioma Cells
Author
최준호
Keywords
Antidepressant; Bcl-xL; Fluoxetine; G alpha i2; Gs alpha; Microarray
Issue Date
2011-08
Publisher
Korean Neurosurgical SOC
Citation
Psychiatry Investigation, 2011, 8(2), P.161-168
Abstract
Objective To analyze both differentially expressed genes and the Bcl-xL protein expression after acute and chronic treatment with fluoxetine in rat C6 glioma cells. Methods C6 glioma cells were cultured for 24 h or 72 h after treatment with 10 mu M fluoxetine, and gene expression patterns were observed using microarray and qRT-PCR. Then, cells were cultured for 6 h, 24 h, 72 h or 96 h after treatment with 10 mu M fluoxetine, and the expression of Bd-xL protein was measured using western blot. Results As determined by microarray, treatment with fluoxetine for 24 h up-regulated 33 genes (including Bcl-xL and NCAM140) and down-regulated 7 genes (including cyclin G-associated kinase). Treatment with fluoxetine for 72 h up-regulated 53 genes (including Gs alpha and Bcl-xL) and down-regulated 77 genes (including Gai2 and annexin V). Based on the qRT-PCR results, there was an increase in Gsa mRNA and a decrease in G alpha i2 mRNA at 72 h in fluoxetine-treated cells as compared to control, a result that was consistent with microarray. We also observed an increase in Bcl-xL mRNA (both at 24 h and at 72 h) in fluoxetine-treated cells as compared to control, demonstrating a tendency to increase gradually. Bcl-xL protein expression increased as the duration of fluoxetine treatment increased. Conclusion These results suggest that chronic treatment with fluoxetine not only initiates the cAMP pathway through inducing Gsa expression but also induces Bcl-xL expression, thus inhibiting apoptosis. Psychiatry Investig 2011;8:161-168
URI
http://psychiatryinvestigation.org/journal/view.php?doi=10.4306/pi.2011.8.2.161
ISSN
1738-3684
DOI
10.4306/pi.2011.8.2.161
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE