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The characterization of gene expression during mouse neural stem cell differentiation in vitro

Title
The characterization of gene expression during mouse neural stem cell differentiation in vitro
Author
채영규
Keywords
Differentiation; Neural stem cells; Cell cycle; BMP; TGF-beta2
Issue Date
2012-01
Publisher
ELSEVIER IRELAND LTD
Citation
NEUROSCIENCE LETTERS; JAN 6 2012, 506 1, p50-p54, 5p.
Abstract
Neural stem cells (NSCs) are tissue-specific, multipotent stem cells that can differentiate into three cell lineages in the central nervous system: neurons, astrocytes and oligodendrocytes. The therapeutic potential of NSCs has fueled attempts to characterize the expression of genes that regulate their fate. In this study, NSCs from embryonic day 15 (E15) mouse embryos were differentiated for 1 (DF-1) or 2 (DF-2) days, and the gene expression patterns in the NSCs and in the DF-1 and DF-2 cells were measured by microarray and real-time RT-PCR. Among the analyzed genes, 1898 genes were up-regulated in the DF-1 and DF-2 cells relative to the NSCs, whereas 1642 genes were down-regulated. The up-regulated genes included Gfap, Smad6, Fst, Tgfb2 and Cdkn2. The down-regulated genes included Ccnb1, Ccnd1 and Ccnd2. We identified gene networks that were associated with BMP and TGF-beta2 signaling pathways using Ingenuity Pathway Analysis. Our results suggest that the differentiation of El 5 NSCs into astrocytes is based on a combinatorial network of various signaling pathways, including cell cycle, BMP and TGF-beta2 signaling. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0304394011014443?_rdoc=1&_fmt=high&_origin=gateway&_docanchor=&md5=b8429449ccfc9c30159a5f9aeaa92ffb
ISSN
0304-3940; 1872-7972
DOI
10.1016/j.neulet.2011.10.046
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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