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dc.contributor.authorJong Wook Hong-
dc.date.accessioned2018-02-13T05:15:25Z-
dc.date.available2018-02-13T05:15:25Z-
dc.date.issued2011-01-
dc.identifier.citationAnalytical chemistry,Vol.83 No.6 [2011],2020-2028en_US
dc.identifier.issn0003-2700-
dc.identifier.issn1520-6882-
dc.identifier.urihttps://pubs.acs.org/doi/abs/10.1021/ac2001737-
dc.description.abstractWe describe a microfluidic device for generating nonlinear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for coflowing multiple aqueous solutions, a main coflow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the coflow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space-saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H 2 O 2 ) concentrations. Graphic Abstract ACS Electronic Supporting Infoen_US
dc.description.sponsorshipS.S., W.Y.S., W.G.L., J.W.H., and AK. designed the study. Y.H.J., S.B.K, W.Y.S., M.K, and S.S. fabricated the devices. S.S., W.Y.S., Y.H.J., and S.B.K. conducted experiments. S.B.K. conducted computer simulations. S.S. and S.B.K. analyzed the data. S.S., H.J.B., W.Y.S., S.B.K., W.G.L., J.W.H., and AK. wrote the paper. All authors edited the manuscript. S.S. and W.Y.S. contributed equally as first authors. This paper was supported by the National Institutes of Health (EB008392; HL092836; EB009196; DE019024), National Science Foundation (DMR0847287), the Institute for Soldier Nanotechnology, the Office of Naval Research, and the US Army Corps of Engineers. W.Y.S. was partially supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-357-D00099). W.G.L. was partially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2010-0005219).en_US
dc.language.isoenen_US
dc.publisherACS AMERICAN CHEMICAL SOCIETYen_US
dc.subjectAnimalsen_US
dc.subjectCell Survivalen_US
dc.subjectdrug effectsen_US
dc.subjectCytological Techniquesen_US
dc.subjectinstrumentationen_US
dc.subjectCytotoxinsen_US
dc.subjecttoxicityen_US
dc.subjectDiffusionen_US
dc.subjectDose-Response Relationshipen_US
dc.subjectDrug, Miceen_US
dc.subjectMicrofluidic Analytical Techniquesen_US
dc.subjectNIH 3T3 Cellsen_US
dc.subjectNonlinear Dynamicsen_US
dc.titleGenerating Non-Linear Concentration Gradients in Microfluidic Devices for Cell Studiesen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac2001737-
dc.relation.page2020-2028-
dc.relation.journalANALYTICAL CHEMISTRY-
dc.contributor.googleauthorSelimovic, S.-
dc.contributor.googleauthorSim, Woo Young-
dc.contributor.googleauthorKim, Sang Bok-
dc.contributor.googleauthorJang, Yun Ho-
dc.contributor.googleauthorLee, Won Gu-
dc.contributor.googleauthorMasoud Khabiry-
dc.contributor.googleauthorBae, Hojae-
dc.contributor.googleauthorSachin Jambovane-
dc.contributor.googleauthorHong, Jong Wook-
dc.contributor.googleauthorAli Khademhosseini-
dc.relation.code2011200599-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjwh-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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