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Generating Non-Linear Concentration Gradients in Microfluidic Devices for Cell Studies

Title
Generating Non-Linear Concentration Gradients in Microfluidic Devices for Cell Studies
Author
Jong Wook Hong
Keywords
Animals; Cell Survival; drug effects; Cytological Techniques; instrumentation; Cytotoxins; toxicity; Diffusion; Dose-Response Relationship; Drug, Mice; Microfluidic Analytical Techniques; NIH 3T3 Cells; Nonlinear Dynamics
Issue Date
2011-01
Publisher
ACS AMERICAN CHEMICAL SOCIETY
Citation
Analytical chemistry,Vol.83 No.6 [2011],2020-2028
Abstract
We describe a microfluidic device for generating nonlinear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for coflowing multiple aqueous solutions, a main coflow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the coflow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space-saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H 2 O 2 ) concentrations. Graphic Abstract ACS Electronic Supporting Info
URI
https://pubs.acs.org/doi/abs/10.1021/ac2001737
ISSN
0003-2700; 1520-6882
DOI
10.1021/ac2001737
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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