A bilayer composite composed of TiO2-incorporated electrospun chitosan membrane and human extracellular matrix sheet as a wound dressing

Title
A bilayer composite composed of TiO2-incorporated electrospun chitosan membrane and human extracellular matrix sheet as a wound dressing
Author
조용우
Keywords
bilayer composite; chitosan; titanium dioxide nanoparticles; human adipose-derived ECM; wound healing; HUMAN ADIPOSE-TISSUE; SCAFFOLDS; REGENERATION; FABRICATION; MECHANISM
Issue Date
2015-09
Publisher
TAYLOR & FRANCIS LTD
Citation
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 26, No. 13, Page. 841-854
Abstract
We designed bilayer composites composed of an upper layer of titanium dioxide (TiO2)-incorporated chitosan membrane and a sub-layer of human adipose-derived extracellular matrix (ECM) sheet as a wound dressing for full-thickness wound healing. The dense and fibrous top layer, which aims to protect the wound from bacterial infection, was prepared by electrospinning of chitosan solution followed by immersion in TiO2 solution. The sponge-like sub-layer, which aims to promote new tissue regeneration, was prepared with acellular ECM derived from human adipose tissue. Using a modified drop plate method, there was a 33.9 and 69.6% reduction in viable Escherichia coli and Staphylococcus aureus on the bilayer composite, respectively. In an in vivo experiment using rats, the bilayer composites exhibited good biocompatibility and provided proper physicochemical and compositional cues at the wound site. Changes in wound size and histological examination of full-thickness wounds showed that the bilayer composites induced faster regeneration of granulation tissue and epidermis with less scar formation, than control wounds. Overall results suggest that the TiO2-incorporated chitosan/ECM bilayer composite can be a suitable candidate as a wound dressing, with an excellent inhibition of bacterial penetration and wound healing acceleration effects.
URI
http://www.tandfonline.com/doi/abs/10.1080/09205063.2015.1061349http://hdl.handle.net/20.500.11754/37023
ISSN
0920-5063; 1568-5624
DOI
10.1080/09205063.2015.1061349
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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