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dc.contributor.author이창호-
dc.date.accessioned2018-02-12T08:22:41Z-
dc.date.available2018-02-12T08:22:41Z-
dc.date.issued2011-07-
dc.identifier.citationJournal of Cell Science, 2011, 124(13), P.2220-2230en_US
dc.identifier.issn0021-9533-
dc.identifier.urihttp://jcs.biologists.org/content/124/13/2220-
dc.description.abstractRecent evidence suggests that inflammation is involved in malignant progression of breast cancer. Sphingosine 1-phosphate (S1P), acting on the G-protein-coupled receptors, is known as a potent inflammatory mediator. In this study, the effect of the inflammatory lipid S1P on the regulation of invasive/migratory phenotypes of MCF10A human breast epithelial cells was investigated to elucidate a causal relationship between inflammation and the control of invasiveness of breast cells. We show that S1P causes induction of matrix metalloproteinase-9 (MMP-9) in vitro and in vivo, and thus enhances invasion and migration. We also show that fos plays a crucial role in the transcriptional activation of MMP-9 by S1P. In addition, activation of extracellular-signal-regulated kinases 1 and 2 (ERK1/2), p38 and alpha serine/threonine-protein kinase (Akt) are involved in the process of S1P-mediated induction of MMP-9 expression and invasion. Activation of the S1P receptor S1P(3) and G(alpha q) are required for S1P-induced invasive/migratory responses, suggesting that the enhancement of S1P-mediated invasiveness is triggered by the specific coupling of S1P(3) to the heterotrimeric G(alpha q) subunit. Activation of phospholipase C-beta(4) and intracellular Ca2+ release are required for S1P-induced MMP-9 upregulation. Taken together, this study demonstrated that S1P regulates MMP-9 induction and invasiveness through coupling of S1P(3) and G(alpha q) in MCF10A cells, thus providing a molecular basis for the crucial role of S1P in promoting breast cell invasion.en_US
dc.language.isoenen_US
dc.publisherCOMPANY OF BIOLOGISTS LTDen_US
dc.subjectS1Pen_US
dc.subjectBreast cell invasionen_US
dc.subjectMMP-9en_US
dc.titleSphingosine 1-phosphate regulates matrix metalloproteinase-9 expression and breast cell invasion through S1P3-Gaq couplingen_US
dc.typeArticleen_US
dc.relation.no13-
dc.relation.volume124-
dc.identifier.doi10.1242/jcs.076794-
dc.relation.page2220-2230-
dc.relation.journalJOURNAL OF CELL SCIENCE-
dc.contributor.googleauthorKim, Eun-Sook-
dc.contributor.googleauthorKim, Jong-Sook-
dc.contributor.googleauthorKim, Sang Geon-
dc.contributor.googleauthorHwang, Sejin-
dc.contributor.googleauthorLee, Chang Ho-
dc.contributor.googleauthorMoon, Aree-
dc.relation.code2011204794-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidjennysue-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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