Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli

Title
Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli
Author
상병인
Keywords
Escherichia coli; Clostridium acetobutylicum; Butyrate; Metabolic Engineering
Issue Date
2011-07
Publisher
SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA
Citation
KOREAN JOURNAL OF CHEMICAL ENGINEERING,Vol.28 No.7 [2011],1587-1592
Abstract
Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.
URI
http://link.springer.com/article/10.1007%2Fs11814-011-0032-6http://hdl.handle.net/20.500.11754/35941
ISSN
0256-1115
DOI
10.1007/s11814-011-0032-6
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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