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dc.contributor.author배옥남-
dc.date.accessioned2018-02-06T01:08:53Z-
dc.date.available2018-02-06T01:08:53Z-
dc.date.issued2015-03-
dc.identifier.citationTOXICOLOGY AND APPLIED PHARMACOLOGY, v. 283, No. 2, Page. 147-155en_US
dc.identifier.issn0041-008X-
dc.identifier.issn1096-0333-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0041008X15000186-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/35479-
dc.description.abstractAllergic contact dermatitis (ACD) is a cell-mediated immune response that involves skin sensitization in response to contact with various allergens. Angiogenesis and lymphangiogenesis both play roles in the allergic sensitization process. Epidermal keratinocytes can produce vascular endothelial growth factor (VEGF) in response to UV irradiation and during wound healing. However, the effect of haptenic chemical allergens on the VEGF production of human keratinocytes, which is the primary contact site of toxic allergens, has not been thoroughly researched. We systematically investigated whether immune-regulatory cytokines and chemical allergens would lead to the production of VEGF in normal human keratinocytes (NHKs) in culture. VEGF production significantly increased when NHKs were treated with IFN-gamma, IL-1 alpha, IL-4, IL-6, IL-17A, IL-22 or TNF alpha. Among the human sensitizers listed in the OECD Test Guideline (TG) 429, we found that CMI/MI, DNCB, 4-phenylenediamine, cobalt chloride, 2-mercaptobenzothiazole, citral, HCA, cinnamic alcohol, imidazolidinyl urea and nickel chloride all significantly upregulated VEGF production in NHKs. In addition, common human haptenic allergens such as avobenzone, formaldehyde and urushiol, also induced the keratinocyte-derived VEGF production. VEGF upregulation by pro-inflammatory stimuli, IFN gamma, DNCB or formaldehyde is preceded by the production of IL-8, an acute inflammatory phase cytokine. Lymphangiogenic VEGF-C gene transcription was significantly increased when NHKs were treated with formaldehyde, DNCB or urushiol, while transcription of VEGF-A and VEGF-B did not change. Therefore, the chemical allergen-induced VEGF upregulation is mainly due to the increase in lymphangiogenic VEGF-C transcription in NHKs. These results suggest that keratinocyte-derived VEGF may regulate the lymphangiogenic process during the skin sensitization process of ACD. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipThis study was partly supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT and Future Planning (Grant No. 2012R1A2A2A01012738) and by the grant (13172MFDS987) from the Ministry of Food and Drug Safety.en_US
dc.language.isoen_USen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.subjectAllergic contact dermatitis (ACD)en_US
dc.subjectNormal human keratinocytesen_US
dc.subjectVEGFen_US
dc.subjectIL-8en_US
dc.subjectOECD TG429en_US
dc.subjectLymphangiogenesisen_US
dc.subjectCONTACT-DERMATITISen_US
dc.subjectIN-VITROen_US
dc.subjectSKIN SENSITIZERSen_US
dc.subjectTNF-ALPHAen_US
dc.subjectLYMPHATIC VESSELSen_US
dc.subjectTRANSGENIC MICEen_US
dc.subjectCELL-LINEen_US
dc.subjectVEGF-Cen_US
dc.subjectINFLAMMATIONen_US
dc.subjectEXPRESSIONen_US
dc.titleChemical allergens stimulate human epidermal keratinocytes to produce lymphangiogenic vascular endothelial growth factoren_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume283-
dc.identifier.doi10.1016/j.taap.2015.01.008-
dc.relation.page147-155-
dc.relation.journalTOXICOLOGY AND APPLIED PHARMACOLOGY-
dc.contributor.googleauthorBae, Ok-Nam-
dc.contributor.googleauthorAhn, Seyeon-
dc.contributor.googleauthorJin, Sun Hee-
dc.contributor.googleauthorHong, Soo Hyun-
dc.contributor.googleauthorLee, Jinyoung-
dc.contributor.googleauthorKim, Eun-Sun-
dc.contributor.googleauthorJeong, Tae Cheon-
dc.contributor.googleauthorChun, Young-Jin-
dc.contributor.googleauthorLee, Ai-Young-
dc.contributor.googleauthorNoh, Minsoo-
dc.relation.code2015001464-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidonbae-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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