Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient

Title
Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient
Author
성기훈
Keywords
NANOLITER PLUGS; LAMINAR-FLOW; DEVICE; GENERATION; COMPLEX; ASSAY; CHIP; CRYSTALLIZATION; OPTIMIZATION; PARAMETERS
Issue Date
2011-03
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v. 83, Page. 1603-1608
Abstract
In this Paper We propose a microfluidic device that is capable Of generating a concentration gradient followed by parallel droplet formation within channels with a simple T-junction geometry. Linear concentration gradient profiles can be obtained based on fluid diffusion under laminar flow. Optimized conditions for generating a linear concentration gradient and parallel droplet formation were investigated using fluorescent dye. The concentration gradient profile under diffusive Mixing was dominated by the flow rate at sample inlets, while parallel droplet formation was affected by the channel geometry at both the inlet and outlet The microfluidic device was experimentally characterized using optimal layout and operating conditions,selected through a design process. Furthermore, in situ enzyme kinetic measurements of the beta- galactosidase-catalyzed hydrolysis of resorufin-beta-D-galactopyranoside were,performed to demonstrate the application potential of our Simple, time-effective, and low sample volume microfluidic device. We expect that, in addition to enzyme kinetics, drug screening and clinical diagnostic tests can be rapidly and accurately performed using this droplet-based microfluidic system.
URI
http://pubs.acs.org/doi/pdf/10.1021/ac102472ahttp://hdl.handle.net/20.500.11754/35347
ISSN
0003-2700; 1520-6882
DOI
10.1021/ac102472a
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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