Identification of Antigenic Proteins in Trichomonas vaginalis

Title
Identification of Antigenic Proteins in Trichomonas vaginalis
Author
류재숙
Keywords
Trichomonas vaginalis; antigenic protein; membrane protein; immunoscreening
Issue Date
2011-03
Publisher
Korean SOC Parasitology
Citation
Korean Journal of Parasitology, 2011, 49(1), P.79-83
Abstract
Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T vaginalis. The T vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T vaginalis into a phage lambda Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, alpha-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T vaginalis. This study led us to identify T vaginalis proteins which may stimulate immune responses by human cells.
URI
http://parasitol.kr/journal/view.php?doi=10.3347/kjp.2011.49.1.79
ISSN
0023-4001; 1738-0006
DOI
10.3347/kjp.2011.49.1.79
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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