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Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum

Title
Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum
Author
채필석
Keywords
Rhodospirillum rubrum; nitrogen metabolism; Blue Native; chromatophore subproteome; amphiphile; Orbitrap
Issue Date
2011-03
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF PROTEOME RESEARCH, v. 10, NO 6, Page. 2703-2714
Abstract
The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
URI
https://pubs.acs.org/doi/abs/10.1021/pr100838x
ISSN
1535-3893
DOI
10.1021/pr100838x
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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