Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 계명찬 | - |
dc.date.accessioned | 2018-02-03T01:12:28Z | - |
dc.date.available | 2018-02-03T01:12:28Z | - |
dc.date.issued | 2011-02 | - |
dc.identifier.citation | THERIOGENOLOGY, v. 75, NO 3, Page. 445-458 | en_US |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0093691X10004954?via%3Dihub | - |
dc.description.abstract | The expression of claudin-1 and -11, tight junctions (TJs) proteins was examined in immature and adult pheasant (Phasianus colchicus) testes. Claudin-1 and -11 cDNA were highly similar to those of human, mice, and chicken. Claudin-1 mRNA and protein (21 kDa) levels in immature testes were higher than those of adult testis. In immature testes until 6 weeks of age, Claudin-1 was found at contacts between adjacent Sertoli cells and between Sertoli cells and germ cells. In adult testis, Claudin-1 was found in early spermatocytes migrating the blood testis barrier (BTB). Blood vessels were positive for claudin-1. Claudin-11 mRNA and protein (21 kDa) increased during adulthood development of testis. In immature testis, Claudin-11 was found in apicolateral contacts between adjacent Sertoli cells, indicating its involvement in cell adhesion in immature testis. In adult testis, strong wavy Claudin-11 immunoreactivity was parallel to basal lamina at the basal part of seminiferous epithelium, indicating that Claudin-11 at the inter-Sertoli TJs may act as a structural element of the BTB. Weak Claudin-1 and -11 immunoreactivity at contacts between Sertoli cells to elongating/elongated spermatids, meiotic germ cells, and basal lamina suggests that they also participate in the cell cell and cell extracellular matrix adhesion in pheasant testis. Testosterone increased claudin-11 mRNA in testis organ culture and Sertoli cell primary culture, suggesting positive regulation of claudin-11 gene by androgen in Sertoli cells of pheasant testis. This is the first report on the claudins expression at BTB in avian testis. (C) 2011 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | The first two authors contributed equally to this work. This study was supported by a grant from the Hanyang University. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.subject | Claudins | en_US |
dc.subject | Tight junction | en_US |
dc.subject | Pheasant testis | en_US |
dc.title | Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 75 | - |
dc.identifier.doi | 10.1016/j.theriogenology.2010.09.012 | - |
dc.relation.page | 445-458 | - |
dc.relation.journal | THERIOGENOLOGY | - |
dc.contributor.googleauthor | Park, C.J. | - |
dc.contributor.googleauthor | Lee, J.E. | - |
dc.contributor.googleauthor | Oh, Y.S. | - |
dc.contributor.googleauthor | Shim, S. | - |
dc.contributor.googleauthor | Nah, W.H. | - |
dc.contributor.googleauthor | Choi, K.J. | - |
dc.contributor.googleauthor | Gye, M.C. | - |
dc.relation.code | 2011209468 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | mcgye | - |
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