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dc.contributor.author이민형-
dc.date.accessioned2018-02-02T01:31:25Z-
dc.date.available2018-02-02T01:31:25Z-
dc.date.issued2011-02-
dc.identifier.citationJOURNAL OF DRUG TARGETING, v. 19, NO 7, Page. 589-596en_US
dc.identifier.issn1061-186X-
dc.identifier.urihttp://www.tandfonline.com/doi/full/10.3109/1061186X.2010.547584-
dc.description.abstractHigh mobility group box-1 A box (HMGB1A) is an anti-inflammatory peptide originating from HMGB1. A previous report demonstrated that recombinant HMGB1A could deliver DNA into cells. Lung epithelial-specific gene delivery is required for the gene therapy of various lung diseases such as acute lung injury. In this study, a lung epithelial-specific DNA carrier was produced by linking the lung epithelial binding peptide (LEBP) to HMGB1A. An LEBP-linked HMGB1A (LEBP-HMGB1A) expression vector, pET21a-LEBP-HMGB1A, was constructed. LEBP-HMGB1A was expressed in BL21 strain and purified by consecutive applications of nickel affinity chromatography and cationic exchange chromatography. In a gel retardation assay, LEBP-HMGB1A completely retarded DNA at a 5:1 weight ratio (peptide: DNA). LEBP-HMGB1A/DNA complexes were prepared at various weight ratios, to which a fixed amount of polyethylenimine (2 kDa, PEI2k) was added to increase the proton buffering effect of the complex. LEBP-HMGB1A had the highest transfection efficiency to L2 lung epithelial cells at a 20: 1 weight ratio (peptide: DNA). At this ratio, LEBPHMGB1A had a higher transfection efficiency than poly-L-lysine (PLL) as well as HMGB1A without LEBP. A cytotoxicity assay showed that LEBP-HMGB1A was not toxic to L2 cells. Therefore, LEBP-HMGB1A may be useful in developing gene therapies for lung diseases.en_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.subjectLung epithelial cellsen_US
dc.subjectgene deliveryen_US
dc.subjecthigh mobility group box-1en_US
dc.subjectrecombinant peptideen_US
dc.titleLung epithelial binding peptide-linked high mobility group box-1: A box for lung epithelial cell-specific delivery of DNAen_US
dc.typeArticleen_US
dc.relation.no7-
dc.relation.volume19-
dc.identifier.doi10.3109/1061186X.2010.547584-
dc.relation.page589-596-
dc.relation.journalJOURNAL OF DRUG TARGETING-
dc.contributor.googleauthorKim, Hyun Ah-
dc.contributor.googleauthorPark, Ji Hwan-
dc.contributor.googleauthorCho, Su Hee-
dc.contributor.googleauthorLee, Minhyung-
dc.relation.code2011204972-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidminhyung-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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