Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최재훈 | - |
dc.date.accessioned | 2018-01-30T07:13:22Z | - |
dc.date.available | 2018-01-30T07:13:22Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 6, Article number 23426 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/srep23426 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/34459 | - |
dc.description.abstract | In adipose tissue, agonists of the beta 3-adrenergic receptor (ADRB3) regulate lipolysis, lipid oxidation, and thermogenesis. The deficiency in the thermogenesis induced by neuroblast differentiation-associated protein AHNAK in white adipose tissue (WAT) of mice fed a high-fat diet suggests that AHNAK may stimulate energy expenditure via development of beige fat. Here, we report that AHNAK deficiency promoted browning and thermogenic gene expression in WAT but not in brown adipose tissue of mice stimulated with the ADRB3 agonist CL-316243. Consistent with the increased thermogenesis, Ahnak(-/-) mice exhibited an increase in energy expenditure, accompanied by elevated mitochondrial biogenesis in WAT depots in response to CL-316243. Additionally, AHNAK-deficient WAT contained more eosinophils and higher levels of type 2 cytokines (IL-4/IL-13) to promote browning of WAT in response to CL-316243. This was associated with enhanced sympathetic tone in the WAT via upregulation of adrb3 and tyrosine hydroxylase (TH) in response to beta-adrenergic activation. CL-316243 activated PKA signalling and enhanced lipolysis, as evidenced by increased phosphorylation of hormone-sensitive lipase and release of free glycerol in Ahnak(-/-) mice compared to wild-type mice. Overall, these findings suggest an important role of AHNAK in the regulation of thermogenesis and lipolysis in WAT via beta-adrenergic signalling. | en_US |
dc.description.sponsorship | This work was supported by the grants from the Korean Ministry of Science, ICT and Future Planning to J.K.S. (KMPC:2013M3A9D5072550). This study was also partially supported by the Research Institute for Veterinary Science, Seoul National University. J.H.S., Y.N.K., S.H.L. and J.K.S. were supported by the Brain Korea 21 Program for Veterinary Science. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | DIET-INDUCED THERMOGENESIS | en_US |
dc.subject | HORMONE-SENSITIVE LIPASE | en_US |
dc.subject | WHITE ADIPOSE-TISSUE | en_US |
dc.subject | ENERGY-EXPENDITURE | en_US |
dc.subject | BEIGE FAT | en_US |
dc.subject | PROTEIN-KINASE | en_US |
dc.subject | BETA-3-ADRENERGIC RECEPTOR | en_US |
dc.subject | ADAPTIVE THERMOGENESIS | en_US |
dc.subject | METABOLIC SYNDROME | en_US |
dc.subject | GENE-EXPRESSION | en_US |
dc.title | AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to beta-adrenergic signalling | en_US |
dc.type | Article | en_US |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1038/srep23426 | - |
dc.relation.page | 23426-23435 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Shin, Jae Hoon | - |
dc.contributor.googleauthor | Lee, Seo Hyun | - |
dc.contributor.googleauthor | Kim, Yo Na | - |
dc.contributor.googleauthor | Kim, Il Yong | - |
dc.contributor.googleauthor | Kim, Youn Ju | - |
dc.contributor.googleauthor | Kyeong, Dong Soo | - |
dc.contributor.googleauthor | Lim, Hee Jung | - |
dc.contributor.googleauthor | Cho, Soo Young | - |
dc.contributor.googleauthor | Choi, Junhee | - |
dc.contributor.googleauthor | Choi, Jae-Hoon | - |
dc.relation.code | 2016012537 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jchoi75 | - |
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