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Environmental Risk Assessment of Toxicity Exposure: High-throughput Expression Profiling

Title
Environmental Risk Assessment of Toxicity Exposure: High-throughput Expression Profiling
Author
황승용
Keywords
Volatile organic compounds (VOCs); Toluene; Ethylbenzene; Xylene; Gene expression profiling; Microarray; Environmental Risk Assessment
Issue Date
2016-03
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Citation
BIOCHIP JOURNAL, v. 10, NO 1, Page. 74-80
Abstract
Recently, volatile organic compounds (VOCs) in outdoor air pollution have led to major public health problems and the identification of specific minimally invasive biomarkers for assessing environmental toxicant exposure has become increasingly important. However, research into the human health effects of inhalation exposure to VOCs remains insufficient. Using a microarray based approach, we identified and validated characteristic mRNA expression profiles in the human whole blood of workers exposed to VOCs (toluene, ethylbenzene, and xylene), which were then compared with genomic level expressions in workers not exposed to the toxicants. We surveyed 141 workers working in a chemical production factory, of which 66 were not exposed to VOCs. We identified 4384 characteristic discernible exposure indicator mRNAs for toluene, 1296 for ethylbenzene, and 5821 for xylene. Using these, we were able to discern those subjects from the control group to a higher accuracy, sensitivity, and specificity than when using urinary biomarkers. The results showed that altered levels of mRNA can be a reliable, novel, and minimally invasive biological indicator of occupational exposure to VOCs. Future research directions should consider the adverse effects of exposure to VOCs on epigenetic regulation.
URI
https://link.springer.com/article/10.1007%2Fs13206-016-0110-2http://hdl.handle.net/20.500.11754/34421
ISSN
1976-0280; 2092-7843
DOI
10.1007/s13206-016-0110-2
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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