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A droplet-based microfluidic immunosensor for high efficiency melamine analysis

Title
A droplet-based microfluidic immunosensor for high efficiency melamine analysis
Author
주재범
Keywords
Melamine; Droplet-based microfluidics; Fluorescence polarization; Milk
Issue Date
2016-02
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v. 80, Page. 182-186
Abstract
We report a droplet-based microfluidic immunosensor for the rapid and accurate detection of melamine, an organic base that has been implicated in widescale adulteration of food products such as milk. Our melamine assay is based on the competitive reaction between native melamine and a melamine-fluorescein isothiocyanate (FITC) conjugate against an anti-hapten antibody. The adoption of fluorescence polarization, allows the quantification of melamine in a more direct and rapid manner than established heterogeneous methods based on liquid chromatography, mass spectrometry, and enzyme-linked immunosorbent assay (ELISA). The detection protocol provides a limit of detection of 300 ppb, which is below the maximum allowable melamine levels (2.5 ppm) defined by the U.S. Food and Drug Administration and the European Commission to a significant extent. (C) 2016 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S095656631530676X?via%3Dihubhttp://hdl.handle.net/20.500.11754/34019
ISSN
0956-5663; 1873-4235
DOI
10.1016/j.bios.2015.12.023
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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