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CYANOBACTYERIA-SPECIFIC ALGICIDAL MECHANISM OF THE ECO-FRIENDLY NAPHTHOQUINONE DERIVATIVE (NQ 2-0)

Title
CYANOBACTYERIA-SPECIFIC ALGICIDAL MECHANISM OF THE ECO-FRIENDLY NAPHTHOQUINONE DERIVATIVE (NQ 2-0)
Other Titles
친환경적인 나프토퀴논 유도체 (NQ 2-0)의 남조류 특이 살조기작
Author
Heon Woo LEE
Alternative Author(s)
이헌우
Advisor(s)
한명수
Issue Date
2017-08
Publisher
한양대학교
Degree
Master
Abstract
Harmful cyanobacteria bloom is an alarming risk for aquatic environment and potentially affect human health. Joo and Han (2017) developed harmful cyanobacteria specific algicide naphthoquinone derivatives 2-0 (NQ 2-0) for the selective remediation. However, the mechanism of selective algicidal effect of NQ 2-0 to the cyanobacteria has not been understood. In this study, I investigated the effect of NQ 2-0 on cyanobacteria, green algae and diatoms in order to clarify selectively of the specific algicidal effect of NQ 2-0. Algicidal effect of NQ 2-0 under light and dark conditions showed that it was 97.41% and 18.98% in Microcystis aeruginosa, respectively. It was not observed under both conditions in Selenastrum capricornutum and Cyclotella sp.. To investigate the effects on photosynthesis due to NQ 2-0 treatment, we measured oxygen evolution rate, chlorophyll (Chl) fluorescence and photosynthetic efficiency (Fv/Fm). The M. aeruginosa treated with the NQ 2-0 exhibited, lower oxygen evolution rate of 60 ~ 150% than that of the control and it showed the negative value while, Chl fluorescence increased by 111 ~ 129% and Fv/Fm decreased by 13 ~ 22%. S. capricornutum was not affected as it was evident with the unaffeced oxygen evolution rate, Chl fluorescence and Fv/Fm. The oxygen evolution rate of Cyclotella sp. was reduced by 33 ~ 100% compared to the control but it had positive value and there was no effect on Chl fluorescence and Fv/Fm. In addition, reactive oxygen species (ROS) generation, antioxidant enzyme activity (SOD, CAT) and lipid peroxidation (malondialdehyde, MDA) were measured to evaluate the stress response on photosynthesis. In the case of M. aeruginosa, ROS occurred after 24 hours of NQ 2-0 was treated. Lipid peroxidation increased after 24 hours, antioxidant enzymes activities increased from 48 hours after NQ 2-0 treated. Both S. capricornutum and Cyclotella sp. remained unaffected since there were no ROS, antioxidant enzyme activity and lipid peroxidation detected. The morphological changes in M. aeruginosa confirmed selective algicidal effect as studied through the TEM. The thylakoid membrane was not observed on 12 hours of the growth, while cytoplasmic vacuolation was observed at 24 hours after NQ 2-0 treated. These results suggest that NQ 2-0 inhibits the photosynthesis of cyanobacteria selectively and the resulting ROS increases lipid peroxidation and destroys the intracellular structure.
URI
http://dcollection.hanyang.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000102919http://hdl.handle.net/20.500.11754/33398
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > LIFE SCIENCE(생명과학과) > Theses (Ph.D.)
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