Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2017-10-20T06:30:40Z | - |
dc.date.available | 2017-10-20T06:30:40Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v. 87, NO 21, Page. 10770-10778 | en_US |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | http://pubs.acs.org/doi/10.1021/acs.analchem.5b02402 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/30148 | - |
dc.description.abstract | Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems. | en_US |
dc.description.sponsorship | This work was supported by Newton International Research Fellowship from Royal Society UK and the Global Research Laboratory Program of the National Research Foundation of Korea (Grant Number K20904000004-11A0500-00410). We thank Mr. Louai Labanieh at the University of California Irvine for the illustration. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | PROTEIN-PROTEIN INTERACTIONS | en_US |
dc.subject | SINGLE-CELL ANALYSIS | en_US |
dc.subject | PICOLITER-VOLUME | en_US |
dc.subject | HODGKINS-DISEASE | en_US |
dc.subject | CANCER | en_US |
dc.subject | APOPTOSIS | en_US |
dc.subject | CASPASE-3 | en_US |
dc.subject | GENERATION | en_US |
dc.subject | GRADIENTS | en_US |
dc.subject | MOLECULE | en_US |
dc.title | 3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation | en_US |
dc.type | Article | en_US |
dc.relation.no | 21 | - |
dc.relation.volume | 87 | - |
dc.identifier.doi | 10.1021/acs.analchem.5b02402 | - |
dc.relation.page | 10770-10778 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.contributor.googleauthor | Kang, Dong-Ku | - |
dc.contributor.googleauthor | Gong, Xiuqing | - |
dc.contributor.googleauthor | Cho, Soongwon | - |
dc.contributor.googleauthor | Kim, Jin-young | - |
dc.contributor.googleauthor | Edel, Joshua B. | - |
dc.contributor.googleauthor | Chang, Soo-Ik | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.contributor.googleauthor | deMello, Andrew J. | - |
dc.relation.code | 2015003190 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.