3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
- Title
- 3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
- Author
- 주재범
- Keywords
- PROTEIN-PROTEIN INTERACTIONS; SINGLE-CELL ANALYSIS; PICOLITER-VOLUME; HODGKINS-DISEASE; CANCER; APOPTOSIS; CASPASE-3; GENERATION; GRADIENTS; MOLECULE
- Issue Date
- 2015-12
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v. 87, NO 21, Page. 10770-10778
- Abstract
- Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.
- URI
- http://pubs.acs.org/doi/10.1021/acs.analchem.5b02402http://hdl.handle.net/20.500.11754/30148
- ISSN
- 0003-2700; 1520-6882
- DOI
- 10.1021/acs.analchem.5b02402
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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