3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation

Title
3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
Author
주재범
Keywords
PROTEIN-PROTEIN INTERACTIONS; SINGLE-CELL ANALYSIS; PICOLITER-VOLUME; HODGKINS-DISEASE; CANCER; APOPTOSIS; CASPASE-3; GENERATION; GRADIENTS; MOLECULE
Issue Date
2015-12
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v. 87, NO 21, Page. 10770-10778
Abstract
Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.
URI
http://pubs.acs.org/doi/10.1021/acs.analchem.5b02402http://hdl.handle.net/20.500.11754/30148
ISSN
0003-2700; 1520-6882
DOI
10.1021/acs.analchem.5b02402
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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