CRISPR/Cas9 system as an innovative genetic engineering tool: Enhancements in sequence specificity and delivery methods

Title
CRISPR/Cas9 system as an innovative genetic engineering tool: Enhancements in sequence specificity and delivery methods
Author
Ramakrishna, Suresh
Keywords
Delivery methods; Genome editing; Reduced off-target effects; Sequence specificity; Therapeutics; CRISPR/Cas9 applications
Issue Date
2015-12
Publisher
ELSEVIER SCIENCE BV
Citation
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, v. 1856, NO 2, Page. 234-243
Abstract
While human gene therapy has gained significant attention for its therapeutic promise, CRISPR/Cas9 technology has made a breakthrough as an efficient genome editing tool by emulating prokaryotic immune defense mechanisms. Although many studies have found that CRISPR/Cas9 technology is more efficient, specific and manipulable than previous generations of gene editing tools, it can be further improved by elevating its overall efficiency in a higher frequency of genome modifications and reducing its off-target effects. Here, we review the development of CRISPR/Cas9 technology, focusing on enhancement of its sequence specificity, reduction of off-target effects and delivery systems. Moreover, we describe recent successful applications of CRISPR/Cas9 technology in laboratory and clinical studies. (C) 2015 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0304419X15000700?via%3Dihubhttp://hdl.handle.net/20.500.11754/30033
ISSN
0304-419X; 0006-3002
DOI
10.1016/j.bbcan.2015.09.003
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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