Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2017-10-13T06:55:23Z | - |
dc.date.available | 2017-10-13T06:55:23Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | PLOS ONE, v. 10, NO 12, Article number e0144491, Page. 1-11 | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144491 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/29627 | - |
dc.description.abstract | Developments of non-viral carriers have headed toward reducing cytotoxicity, which results from the use of conventional gene carriers, and enhancing gene delivery efficiency. Cys(D-R9)-Cys repeated reducible poly(oligo-D-arginine) (rPOA) is one of the most efficient non-viral carriers for gene therapy; however, while its efficiency has been verified in the lung and brain, it is necessary to confirm its activity in each organ or tissue since there are differences of gene carrier susceptibility to among tissue types. We therefore tested the compatibility of rPOA in cardiac tissue by in vitro or in vivo experiments and confirmed its high transfection efficiency and low cytotoxicity. Moreover, substantial regenerative effects were observed following transfection with rPOA/pVEGF expression vector complexes (79% decreased infarct size) compared to polyethyleneimine (PEI) (34% decreased infarct size) in a rat myocardial infarction (MI) model. These findings suggest that rPOA efficiently enables DNA transfection in cardiac tissue and can be used as a useful non-viral therapeutic gene carrier for gene therapy in ischemic heart disease. | en_US |
dc.description.sponsorship | This study was supported by a grant from the Korea Healthcare Technology Research & Development Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (Nos. A085136 and A102064), the Mid-career Researcher Program through NRF grant funded by the MEST, Republic of Korea (No. 2015R1A2A2A01002731) and the Cardiovascular Research Center, Seoul, Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | PUBLIC LIBRARY SCIENCE | en_US |
dc.subject | MYOCARDIAL-INFARCTION | en_US |
dc.subject | VEGF PLASMID | en_US |
dc.subject | THERAPY | en_US |
dc.subject | ISCHEMIA | en_US |
dc.subject | GENOME | en_US |
dc.subject | OXYGEN | en_US |
dc.title | Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression | en_US |
dc.type | Article | en_US |
dc.relation.no | 12 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1371/journal.pone.0144491 | - |
dc.relation.page | 1-11 | - |
dc.relation.journal | PLOS ONE | - |
dc.contributor.googleauthor | Woo, Jongsu | - |
dc.contributor.googleauthor | Bae, Seong-Ho | - |
dc.contributor.googleauthor | Kim, Bokyoung | - |
dc.contributor.googleauthor | Park, Jin Sil | - |
dc.contributor.googleauthor | Jung, Subin | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Kim, Yong-Hee | - |
dc.contributor.googleauthor | Choi, Donghoon | - |
dc.relation.code | 2015008685 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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