Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이근용 | - |
dc.date.accessioned | 2017-09-29T00:06:36Z | - |
dc.date.available | 2017-09-29T00:06:36Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | NANOSCALE, v. 7, NO 47, Page. 20095-20104 | en_US |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.issn | 2040-3372 | - |
dc.identifier.uri | http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR06903C#!divAbstract | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/29488 | - |
dc.description.abstract | Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R-9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R(9)G(n)) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R(9)G(n)-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R(9)G(n)-chitosan and siRNA. However, R(9)G(10)-chitosan was much more effective in delivering genes both in vitro and in vivo compared with nonmodified chitosan (without the peptide) and R-9-chitosan (without the spacer arm). Chitosan derivatives modified with oligoarginine containing a spacer arm can be considered as potential delivery vehicles for various genes. | en_US |
dc.description.sponsorship | This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013R1A2A2A03010055). M. C. also acknowledges the NRF for partial financial support (NRF-2012M3A9D1054451). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | SIRNA DELIVERY | en_US |
dc.subject | GENE DELIVERY | en_US |
dc.subject | RNA INTERFERENCE | en_US |
dc.subject | IN-VITRO | en_US |
dc.subject | ARGININE | en_US |
dc.subject | PROGRESS | en_US |
dc.subject | THERAPY | en_US |
dc.subject | VECTORS | en_US |
dc.subject | POLYMER | en_US |
dc.subject | SYSTEMS | en_US |
dc.title | The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment | en_US |
dc.type | Article | en_US |
dc.relation.no | 47 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1039/c5nr06903c | - |
dc.relation.page | 20095-20104 | - |
dc.relation.journal | NANOSCALE | - |
dc.contributor.googleauthor | Jeong, Eun Ju | - |
dc.contributor.googleauthor | Choi, Moonhwan | - |
dc.contributor.googleauthor | Lee, Jangwook | - |
dc.contributor.googleauthor | Rhim, Taiyoun | - |
dc.contributor.googleauthor | Lee, Kuen Yong | - |
dc.relation.code | 2015000055 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | leeky | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.