Analysis of the Microbial Community in an Acidic Hollow-Fiber Membrane Biofilm Reactor (Hf-MBfR) Used for the Biological Conversion of Carbon Dioxide to Methane

Title
Analysis of the Microbial Community in an Acidic Hollow-Fiber Membrane Biofilm Reactor (Hf-MBfR) Used for the Biological Conversion of Carbon Dioxide to Methane
Author
상병인
Keywords
SP-NOV; METHANOTHERMOBACTER-MARBURGENSIS; HYDROGENOTROPHIC METHANOGENESIS; ANAEROBIC OXIDATION; CONTINUOUS-CULTURE; CATTLE MANURE; SPOROMUSA; PERFORMANCE; BACTERIA; WASTE
Issue Date
2015-12
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v. 10, NO 12, Article number e0144999, Page. 1-16
Abstract
Hydrogenotrophic methanogens can use gaseous substrates, such as H-2 and CO2, in CH4 production. H-2 gas is used to reduce CO2. We have successfully operated a hollow-fiber membrane biofilm reactor (Hf-MBfR) for stable and continuous CH4 production from CO2 and H-2. CO2 and H-2 were diffused into the culture medium through the membrane without bubble formation in the Hf-MBfR, which was operated at pH 4.5-5.5 over 70 days. Focusing on the presence of hydrogenotrophic methanogens, we analyzed the structure of the microbial community in the reactor. Denaturing gradient gel electrophoresis (DGGE) was conducted with bacterial and archaeal 16S rDNA primers. Real-time qPCR was used to track changes in the community composition of methanogens over the course of operation. Finally, the microbial community and its diversity at the time of maximum CH4 production were analyzed by pyrosequencing methods. Genus Methanobacterium, related to hydrogenotrophic methanogens, dominated the microbial community, but acetate consumption by bacteria, such as unclassified Clostridium sp., restricted the development of acetoclastic methanogens in the acidic CH4 production process. The results show that acidic operation of a CH4 production reactor without any pH adjustment inhibited acetogenic growth and enriched the hydrogenotrophic methanogens, decreasing the growth of acetoclastic methanogens.
URI
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144999http://hdl.handle.net/20.500.11754/29456
ISSN
1932-6203
DOI
10.1371/journal.pone.0144999
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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