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dc.contributor.author황승용-
dc.date.accessioned2017-09-27T05:19:24Z-
dc.date.available2017-09-27T05:19:24Z-
dc.date.issued2015-12-
dc.identifier.citationMITOCHONDRIAL DNA PART A, v. 28, NO 3, Page. 379-382en_US
dc.identifier.issn2470-1394-
dc.identifier.issn2470-1408-
dc.identifier.urihttp://www.tandfonline.com/doi/full/10.3109/19401736.2015.1126831-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/29425-
dc.description.abstractMackerel is marketed at prices according to the species type, Scomber japonicus and Scomber scombrus. Distinguishing these two species with the naked eye is difficult, and their differentiation becomes more difficult after they are processed by cooking, thereby leading to counterfeiting issues. Thus, in this study, we developed a method to differentiate S. japonicus from S. scombrus by detecting polymorphisms in mitochondrial 16 s rRNA gene by using fluorescence melting curve analysis and locked nucleic acid probes. Our method could distinguish S. japonicus from S. scombrus in a single experiment by using a single probe. The probes developed matched exactly with S. japonicus and had a melting temperature of 64 degrees C. However, the probes were mismatched with S. scombrus, resulting in a lower melting temperature of 46 degrees C. The high specificity of the locked nucleic acid probes resulted in this large difference in the melting temperatures.en_US
dc.description.sponsorshipThere is no conflict of interest to disclose. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST; No. 2012R1A2A2A03045008).en_US
dc.language.isoenen_US
dc.publisherINFORMA HEALTHCAREen_US
dc.subjectFluorescence melting curve analysisen_US
dc.subjectLocked nucleic aciden_US
dc.subjectScomber japonicusen_US
dc.subjectScomber scombrusen_US
dc.subjectspecies identificationen_US
dc.titleDifferentiation of Scomber japonicus from Scomber scombrus by using a single locked nucleic acid probeen_US
dc.typeArticleen_US
dc.identifier.doi10.3109/19401736.2015.1126831-
dc.relation.page1-4-
dc.relation.journalMITOCHONDRIAL DNA-
dc.contributor.googleauthorAhn, Jeong Jin-
dc.contributor.googleauthorKim, Youngjoo-
dc.contributor.googleauthorHong, Ji Young-
dc.contributor.googleauthorKim, Gi Won-
dc.contributor.googleauthorKim, Seol Young-
dc.contributor.googleauthorHwang, Seung Yong-
dc.relation.code2015001379-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidsyhwang-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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