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Differentiation of Scomber japonicus from Scomber scombrus by using a single locked nucleic acid probe

Title
Differentiation of Scomber japonicus from Scomber scombrus by using a single locked nucleic acid probe
Author
황승용
Keywords
Fluorescence melting curve analysis; Locked nucleic acid; Scomber japonicus; Scomber scombrus; species identification
Issue Date
2015-12
Publisher
INFORMA HEALTHCARE
Citation
MITOCHONDRIAL DNA PART A, v. 28, NO 3, Page. 379-382
Abstract
Mackerel is marketed at prices according to the species type, Scomber japonicus and Scomber scombrus. Distinguishing these two species with the naked eye is difficult, and their differentiation becomes more difficult after they are processed by cooking, thereby leading to counterfeiting issues. Thus, in this study, we developed a method to differentiate S. japonicus from S. scombrus by detecting polymorphisms in mitochondrial 16 s rRNA gene by using fluorescence melting curve analysis and locked nucleic acid probes. Our method could distinguish S. japonicus from S. scombrus in a single experiment by using a single probe. The probes developed matched exactly with S. japonicus and had a melting temperature of 64 degrees C. However, the probes were mismatched with S. scombrus, resulting in a lower melting temperature of 46 degrees C. The high specificity of the locked nucleic acid probes resulted in this large difference in the melting temperatures.
URI
http://www.tandfonline.com/doi/full/10.3109/19401736.2015.1126831http://hdl.handle.net/20.500.11754/29425
ISSN
2470-1394; 2470-1408
DOI
10.3109/19401736.2015.1126831
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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