Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

Title
Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy
Authors
하태경
Keywords
Vertical sleeve gastrectomy; Proteome; Metabolome; Gluconeogenesis
Issue Date
2015-11
Publisher
SPRINGER
Citation
OBESITY SURGERY, v. 25, NO 11, Page. 2002-2010
Abstract
We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 +/- 5.6 mg/dL; VSG, 138.5 +/- 3.7 mg/dL) decreased while random insulin (sham, 0.45 +/- 0.16 mu g/L; VSG, 1.05 +/- 0.18 mu g/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. This study proves that VSG suppresses hepatic glucose production.
URI
https://link.springer.com/article/10.1007%2Fs11695-015-1636-4http://hdl.handle.net/20.500.11754/29141
ISSN
0960-8923; 1708-0428
DOI
http://dx.doi.org/10.1007/s11695-015-1636-4
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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