Characterization of a solvent, surfactant and temperature-tolerant laccase from Pleurotus sp MAK-II and its dye decolorizing property

Title
Characterization of a solvent, surfactant and temperature-tolerant laccase from Pleurotus sp MAK-II and its dye decolorizing property
Authors
KUTZNER, ARNE HOLGER
Keywords
Congo Red; Laccase; Pleurotus sp.; Remazol Brilliant Blue R; Violuric acid
Issue Date
2015-11
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS, v. 37, NO 12, Page. 2403-2409
Abstract
Objective Laccase is industrially important but a major challenge is the production of an ideal laccase with suitable physicochemical properties to tolerate temperature, surfactants, metal ions and solvents towards its potential application in bioremediation. Results A laccase with a molecular mass of 43 kDa was purified from Pleurotus sp. MAK-II. It was optimally active at pH 4.5 and 60 degrees C using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as substrate. The laccase was susceptible to NaN3 and NaCN. Activity was strongly enhanced by Cu2+, Mg2+ and Ca2+. The purified laccase showed stability towards various surfactants and solvents and decolorized, in the presence of violuric acid as redox mediator, the diazo dye Congo Red and the anthraquinone dye Remazol Brilliant Blue R to the extent of 96 and 72 %, respectively. Conclusion The ideal physicochemical properties of Pleurotus sp. MAK-II-derived laccase suggest that it could be effectively used in the textile dye industry.
URI
https://link.springer.com/article/10.1007%2Fs10529-015-1937-7http://hdl.handle.net/20.500.11754/29116
ISSN
0141-5492; 1573-6776
DOI
http://dx.doi.org/10.1007/s10529-015-1937-7
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COLLEGE OF ENGINEERING[S](공과대학) > ETC
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