Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Klaus Heese | - |
dc.date.accessioned | 2017-09-08T07:02:57Z | - |
dc.date.available | 2017-09-08T07:02:57Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, NO 37(12), Page. 2403-2409 | en_US |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.issn | 1573-6776 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs10529-015-1937-7 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/29006 | - |
dc.description.abstract | Objective Laccase is industrially important but a major challenge is the production of an ideal laccase with suitable physicochemical properties to tolerate temperature, surfactants, metal ions and solvents towards its potential application in bioremediation. Results A laccase with a molecular mass of 43 kDa was purified from Pleurotus sp. MAK-II. It was optimally active at pH 4.5 and 60 °C using ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as substrate. The laccase was susceptible to NaN3 and NaCN. Activity was strongly enhanced by Cu2+, Mg2+ and Ca2+. The purified laccase showed stability towards various surfactants and solvents and decolorized, in the presence of violuric acid as redox mediator, the diazo dye Congo Red and the anthraquinone dye Remazol Brilliant Blue R to the extent of 96 and 72 %, respectively. Conclusion The ideal physicochemical properties of Pleurotus sp. MAK-II-derived laccase suggest that it could be effectively used in the textile dye industry. | en_US |
dc.description.sponsorship | The authors thank the University of Madras Grants Commission for providing the scholarship to A.M. through CSIR-SRF and Dr. N. Anand, CAS in Botany, University of Madras, for providing the adequate laboratory facility to carry out the research. This study was supported by Hanyang University. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER | en_US |
dc.subject | Congo Red | en_US |
dc.subject | Laccase | en_US |
dc.subject | Pleurotus sp. | en_US |
dc.subject | Remazol Brilliant Blue R | en_US |
dc.subject | Violuric acid | en_US |
dc.title | Characterization of a solvent, surfactant and temperaturetolerant laccase from Pleurotus sp. MAK-II and its dye decolorizing property | en_US |
dc.type | Article | en_US |
dc.relation.no | 37(12) | - |
dc.identifier.doi | 10.1007/s10529-015-1937-7 | - |
dc.relation.page | 2403-2409 | - |
dc.relation.journal | BIOTECHNOLOGY LETTERS | - |
dc.contributor.googleauthor | Manavalan, Arulmani | - |
dc.contributor.googleauthor | Manavalan, Tamilvendan | - |
dc.contributor.googleauthor | Murugesan, Kumarasamy | - |
dc.contributor.googleauthor | Kutzner, Arne | - |
dc.contributor.googleauthor | Thangavelu, Kalaichelvan P. | - |
dc.contributor.googleauthor | Heese, Klaus | - |
dc.relation.code | 2015003603 | - |
dc.sector.campus | S | - |
dc.sector.daehak | Graduate School of Biomedical Science & Engineering[S] | - |
dc.identifier.pid | klaus | - |
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