125 7

Enhanced Detection of Single Cell-secreted Proteins using Fluorescent Immunochemistry on Protein G Terminated Glass Surface

Title
Enhanced Detection of Single Cell-secreted Proteins using Fluorescent Immunochemistry on Protein G Terminated Glass Surface
Author
최종훈
Keywords
microwell array; antibody’s orientation; single cell analysis; secreted cytokine; protein-G-terminated surface
Issue Date
2015-11
Publisher
DOVE MEDICAL PRESS LTD
Citation
INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, Page. 7197-7205
Abstract
We present an evaluation of protein-G-terminated glass slides that may contain a suitable substrate for aligning the orientation of antibodies to obtain better binding moiety to the target antigen. The results of the protein-G-terminated slides were compared with those obtained with epoxy-based slides to evaluate signal enhancement for human immunoglobulin G (IgG) targets, and an increase in the average fluorescence intensity was observed for the lowest measurable amount of IgG target in the assay using protein-G-terminated slides. Applying this strategy for signal amplification to single-cell assays improves the limits of detection for human IgG protein and cytokines (interleukin-2 and interferon-γ) captured from hybridomas. Our data indicate that protein-G-terminated slides have a higher binding capacity for antigens and have better spot-to-spot consistency than that of traditional epoxy-based slides. These properties would be beneficial in the detection of fine amounts of single-cell-secreted proteins, which may provide key insights into cell–cell communication and immune responses.
URI
https://www.dovepress.com/enhanced-detection-of-single-cell-secreted-proteins-using-a-fluorescen-peer-reviewed-article-IJNhttp://hdl.handle.net/20.500.11754/28710
ISSN
1176-9114; 1178-2013
DOI
10.2147/IJN.S92596
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
Enhanced Detection of Single Cell-secreted Proteins using Fluorescent Immunochemistry on Protein G Terminated Glass Surface.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE