Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2017-08-09T06:04:58Z | - |
dc.date.available | 2017-08-09T06:04:58Z | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | COLLOIDS AND SURFACES B-BIOINTERFACES, v. 134, Page. 196-203 | en_US |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.issn | 1873-4367 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0927776515300370?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/28415 | - |
dc.description.abstract | Regeneration of healthy endothelium onto vascular graft materials is imperative for prevention of intimal hyperplasia and thrombogenesis. In this study, we investigated the effect of collagen type IV (COL-IV) immobilized onto electrospun nanofibers on modulation of endothelial cell (EC) function, as a potential signal to rapid endothelialization of vascular grafts. COL-IV is assembled in basement membrane underneath intimal layer and regulates morphogenesis of blood vessels. For immobilization of COL-IV, poly(L-lactic acid) (PLLA) nanofibers (PL) were prepared as a model vascular graft substrate, onto which acrylic acid (AAc) was then grafted by using gamma-ray irradiation. AAc graft was dependent on irradiation doses and AAc concentrations, which allowed us to select the condition of 5% (v/v) AAc and 10 kGy for further conjugation of COL-IV. COL-IV immobilization was proportionally controlled as a function of its concentration. Atomic force microscope (AFM) analysis qualitatively supported immobilization of COL-IV, demonstrating increase in root mean square roughness of the PL from 665.37 +/- 13.20 nm to 1440.74 +/- 33.24. However, the Young's modulus of nanofibers was retained as approximately 1 MPa, regardless of surface modification. The number of ECs attached on the nanofibers with immobilized COLIV was significantly increased by 5 times (1052 +/- 138 cells/mm(2## from pristine PL #2341 +/- 41 cells/mm#2#). In addition, the effect of immobilized COL-IV was profound for enhancing proliferation and up-regulation of markers implicated in rapid endothelialization. Collectively, our results suggest that COL-IV immobilized onto electrospun PLLA nanofibers may serve as a promising instructive cue used in vascular graft materials. #C) 2015 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government, Radiation Technology R&D program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (2014M2A2A6020989) and the Korea Health technology R&D Project, Ministry of Health & Welfare, republic of Korea (A121044). Y. Heo and Y.B. Lee are fellowship awardees by BK21 PLUS program made to BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Electrospinning | en_US |
dc.subject | Surface modification | en_US |
dc.subject | Endothelialization | en_US |
dc.subject | Vascular graft | en_US |
dc.subject | Collagen type IV | en_US |
dc.title | Effect of immobilized collagen type IV on biological properties of endothelial cells for the enhanced endothelialization of synthetic vascular graft materials | en_US |
dc.type | Article | en_US |
dc.relation.volume | 134 | - |
dc.identifier.doi | 10.1016/j.colsurfb.2015.07.003 | - |
dc.relation.page | 196-203 | - |
dc.relation.journal | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.contributor.googleauthor | Heo, Yunhoe | - |
dc.contributor.googleauthor | Shin, Young Min | - |
dc.contributor.googleauthor | Lee, Yu Bin | - |
dc.contributor.googleauthor | Lim, Youn Mook | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.relation.code | 2015001660 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
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