139 0

CD44 regulates cell proliferation, migration, and invasion via modulation of c-Src transcription in human breast cancer cells

Title
CD44 regulates cell proliferation, migration, and invasion via modulation of c-Src transcription in human breast cancer cells
Author
신인철
Keywords
CD44; c-Src; c-Jun; GSK-3 beta; Breast cancer
Issue Date
2015-09
Publisher
ELSEVIER SCIENCE INC
Citation
CELLULAR SIGNALLING, v. 27, NO 9, Page. 1882-1894
Abstract
CD44 was recently identified as a cancer initiation marker on the cell membrane. The cytoplasmic tail of CD44 is known to bind ERM (ezrin, radixin, moesin) proteins, cytoskeletal proteins like ankyrin, and the non-receptor tyrosine kinase c-Src. CD44 transmits its oncogenic signaling via c-Src and its downstream effectors. To investigate the role of CD44 in breast cancer cells, we generated CD44 knock-down cells via retroviral delivery of shRNA against CD44. We found that silencing of CD44 decreased the proliferation, migration, and invasion of breast cancer cells. The expression and activity of cell migration-related proteins, including c-Src, paxillin, and FAK were decreased by CD44 silencing. We also found that the c-Jun protein level was negatively regulated via induction of a GSK-3 beta-dependent degradation pathway in CD44 knock-down cells. The expression level of Sp1, a target gene product of c-Jun, was also decreased in these cells. Finally, CD44 knock-down suppressed both mRNA and protein levels of c-Sic and its downstream MAPK pathway as a result of down-regulation of Sp1 as a transcription factor for c-Src. Collectively, these results indicate that biological changes induced by CD44 silencing are mediated by cumulative down-regulation of c-Jun, Sp1, and c-Src in human breast cancer cells. (C) 2015 Elsevier Inc All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0898656815001503http://hdl.handle.net/20.500.11754/27679
ISSN
0898-6568; 1873-3913
DOI
10.1016/j.cellsig.2015.05.002
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE