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dc.contributor.author채영규-
dc.date.accessioned2017-06-05T06:07:59Z-
dc.date.available2017-06-05T06:07:59Z-
dc.date.issued2015-09-
dc.identifier.citationBIOCHIP JOURNAL, v. 9, NO 3, Page. 182-193en_US
dc.identifier.issn1976-0280-
dc.identifier.issn2092-7843-
dc.identifier.urihttps://link.springer.com/article/10.1007/s13206-015-9302-4-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/27615-
dc.description.abstractNeural differentiation involves complex changes of gene expression patterns, which are controlled by chromatin remodeling that promotes or inhibits neurogenesis and gliogenesis. To study the roles of the ubiquitously transcribed tetratricopeptide repeat on chromosome X (UTX) during neuronal differentiation, we performed gene expression analysis in gene knock down experiments using an artificial miRNA technique. Microarray analysis found that a total of 919 genes were differentially altered in the UTX-KD embryonic carcinoma (NCCIT) cells, and a total of 964 genes in the UTX-KD embryoid bodies (EBs) by 2.0 fold cut off value. Gene ontology analysis revealed the association of cell adhesion related genes were enhanced by UTX-KD. Morphological analysis also showed more attached neurites during differentiation with UTX-KD cells. Differentiated neurons were characterized as GABAergic neurons expressing typical neuronal markers, TU-20 and GAD65. Collectively, our data suggest that knocking down of UTX enhances cell attachment by enhancing related gene expressions and thereby promotes early neuronal cell differentiation.en_US
dc.description.sponsorshipThis research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2013 R1A1A3011026 to K.H.J. and No2011-0030049 to Y.G.C.).en_US
dc.language.isoenen_US
dc.publisherKOREAN BIOCHIP SOCIETY-KBCSen_US
dc.subjectNeuronal differentiationen_US
dc.subjectEmbryonic carcinoma cellsen_US
dc.subjectChromatin modificationen_US
dc.subjectUTXen_US
dc.subjectMicroarray analysisen_US
dc.titleKnocking Down of UTX in NCCIT Cells Enhance Cell Attachment and Promote Early Neuronal Cell Differentiationen_US
dc.typeArticleen_US
dc.relation.no3-
dc.relation.volume9-
dc.identifier.doi10.1007/s13206-015-9302-4-
dc.relation.page182-193-
dc.relation.journalBIOCHIP JOURNAL-
dc.contributor.googleauthorMandal, Chanchal-
dc.contributor.googleauthorJung, Kyoung Hwa-
dc.contributor.googleauthorKang, Sung Chul-
dc.contributor.googleauthorChoi, Mi Ran-
dc.contributor.googleauthorPark, Kyoung Sun-
dc.contributor.googleauthorChung, Ii Yup-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2015006188-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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