Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 고성호 | - |
dc.date.accessioned | 2017-05-23T04:56:54Z | - |
dc.date.available | 2017-05-23T04:56:54Z | - |
dc.date.issued | 2015-09 | - |
dc.identifier.citation | PLOS ONE, v. 10, NO 9, Page. 1-16 | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138724 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/27407 | - |
dc.description.abstract | Adult mammalian brain can be plastic after injury and disease. Therefore, boosting endogenous repair mechanisms would be a useful therapeutic approach for neurological disorders. Isoxazole-9 (Isx-9) has been reported to enhance neurogenesis from neural stem/progenitor cells (NSPCs). However, the effects of Isx-9 on other types of progenitor/precursor cells remain mostly unknown. In this study, we investigated the effects of Isx-9 on the three major populations of progenitor/precursor cells in brain: NSPCs, oligodendrocyte precursor cells (OPCs), and endothelial progenitor cells (EPCs). Cultured primary NSPCs, OPCs, or EPCs were treated with various concentrations of Isx-9 (6.25, 12.5, 25, 50 mu M), and their cell numbers were counted in a blinded manner. Isx-9 slightly increased the number of NSPCs and effectively induced neuronal differentiation of NSPCs. However, Isx-9 significantly decreased OPC number in a concentration-dependent manner, suggesting cytotoxicity. Isx-9 did not affect EPC cell number. But in a matrigel assay of angiogenesis, Isx-9 significantly inhibited tube formation in outgrowth endothelial cells derived from EPCs. This potential anti-tube-formation effect of Isx-9 was confirmed in a brain endothelial cell line. Taken together, our data suggest that mechanisms and targets for promoting stem/progenitor cells in the central nervous system may significantly differ between cell types. | en_US |
dc.description.sponsorship | Work was supported in part by grants from the National Institutes of Health (P01-NS55104), the Uehara Memorial Foundation, and the Glaxo-Smith-Kline-Harvard-Stem-Cell-Institute consortium. Salaries for authors with primary appointments with Glaxo-Smith-Kline (JZ, HL, JCH, TTC, and JDM) were paid by Glaxo-Smith-Kline. JZ, HL, JCH, TTC, and JDM participated in study design and data analysis. | en_US |
dc.language.iso | en | en_US |
dc.publisher | PUBLIC LIBRARY SCIENCE | en_US |
dc.subject | STROKE RECOVERY | en_US |
dc.subject | SMALL-MOLECULE | en_US |
dc.subject | STEM-CELLS | en_US |
dc.subject | BRAIN | en_US |
dc.subject | ANGIOGENESIS | en_US |
dc.subject | NEUROGENESIS | en_US |
dc.subject | PROLIFERATION | en_US |
dc.subject | TRANSLATION | en_US |
dc.subject | THERAPY | en_US |
dc.subject | FATE | en_US |
dc.title | Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1371/journal.pone.0138724 | - |
dc.relation.page | 1-16 | - |
dc.relation.journal | PLOS ONE | - |
dc.contributor.googleauthor | Koh, Seong-Ho | - |
dc.contributor.googleauthor | Liang, Anna C. | - |
dc.contributor.googleauthor | Takahashi, Yoko | - |
dc.contributor.googleauthor | Maki, Takakuni | - |
dc.contributor.googleauthor | Shindo, Akihiro | - |
dc.contributor.googleauthor | Osumi, Noriko | - |
dc.contributor.googleauthor | Zhao, Jing | - |
dc.contributor.googleauthor | Lin, Hong | - |
dc.contributor.googleauthor | Holder, Julie C. | - |
dc.contributor.googleauthor | Chuang, Tsu Tshen | - |
dc.relation.code | 2015008685 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ksh213 | - |
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