Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Wook Hong | - |
dc.date.accessioned | 2017-05-22T01:52:49Z | - |
dc.date.available | 2017-05-22T01:52:49Z | - |
dc.date.issued | 2015-09 | - |
dc.identifier.citation | NANOSCALE, v. 8, NO 4, Page. 1944-1951 | en_US |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.issn | 2040-3372 | - |
dc.identifier.uri | http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C5NR06230F#!divAbstract | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/27363 | - |
dc.description.abstract | Many countries categorize the causative agents of severe infectious diseases as high-risk pathogens. Given their extreme infectivity and potential to be used as biological weapons, a rapid and sensitive method for detection of high-risk pathogens (e.g., Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Vaccinia virus) is highly desirable. Here, we report the construction of a novel detection platform comprising two units: (1) magnetic beads separately conjugated with multiple capturing antibodies against four different high-risk pathogens for simple and rapid isolation, and (2) genetically engineered apoferritin nanoparticles conjugated with multiple quantum dots and detection antibodies against four different high-risk pathogens for signal amplification. For each high-risk pathogen, we demonstrated at least 10-fold increase in sensitivity compared to traditional lateral flow devices that utilize enzyme-based detection methods. Multiplexed detection of high-risk pathogens in a sample was also successful by using the nanoconstructs harboring the dye molecules with fluorescence at different wavelengths. We ultimately envision the use of this novel nanoprobe detection platform in future applications that require highly sensitive on-site detection of high-risk pathogens. | en_US |
dc.description.sponsorship | The authors appreciate Dr Jun Ho Jeon at the Division of High-risk Pathogen Research, Center for Infectious Diseases, Korea National Institute of Health for generously providing inactivated high-risk pathogens. This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education (grant number: 2013R1A1A1012653) and the grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C3266). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | REAL-TIME PCR | en_US |
dc.subject | PUBLIC-HEALTH MANAGEMENT | en_US |
dc.subject | FRANCISELLA-TULARENSIS | en_US |
dc.subject | YERSINIA-PESTIS | en_US |
dc.subject | BACILLUS-ANTHRACIS | en_US |
dc.subject | BIOLOGICAL WEAPON | en_US |
dc.subject | RAPID-DETECTION | en_US |
dc.subject | NATURAL FOCI | en_US |
dc.subject | TULAREMIA | en_US |
dc.subject | ASSAY | en_US |
dc.title | Engineered Nanoconstructs for the Multiplexed and Sensitive Detection of High-risk Pathogens | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 8 | - |
dc.identifier.doi | 10.1039/c5nr06230f | - |
dc.relation.page | 1944-1951 | - |
dc.relation.journal | NANOSCALE | - |
dc.contributor.googleauthor | Seo, Youngmin | - |
dc.contributor.googleauthor | Kim, Ji-eun | - |
dc.contributor.googleauthor | Jeong, Yoon | - |
dc.contributor.googleauthor | Lee, Kwan Hong | - |
dc.contributor.googleauthor | Hwang, Jangsun | - |
dc.contributor.googleauthor | Hong, Jongwook | - |
dc.contributor.googleauthor | Park, Hansoo | - |
dc.contributor.googleauthor | Choi, Jonghoon | - |
dc.relation.code | 2015000055 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jwh | - |
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