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Percutaneous Needle Puncture Technique to Create a Rabbit Model with Traumatic Degenerative Disk Disease

Title
Percutaneous Needle Puncture Technique to Create a Rabbit Model with Traumatic Degenerative Disk Disease
Author
김동원
Keywords
Degeneration; Intervertebral disk disease; Percutaneous technique; Rabbit model
Issue Date
2015-08
Publisher
ELSEVIER SCIENCE INC
Citation
WORLD NEUROSURGERY, v. 84, NO 2, Page. 438-445
Abstract
BACKGROUND: Several studies have been conducted to identify the pathogenesis of and manage disk degeneration. To further this research, reliable animal models of disk degeneration are required. In the present study, a percutaneous technique is used to create a rabbit model of degenerative disk disease, and the reproducibility and efficacy of this technique is reported. METHODS: Ten mature male New Zealand white rabbits were included in the present study. The intervertebral disk was injured by a percutaneous technique at the L2-L3, L3-L4, and L4-L5 levels. The center of the disk was identified by C-arm guidance, A 19-gauge spinal needle with a 10-mL syringe was inserted into the center of the disk, and negative pressure was applied. Radiographs including magnetic resonance imaging (MRI) with T-2 and lateral x-rays were collected at 1, 4, 9, 15, and 20 weeks. Degeneration vvas examined using histology at 24 weeks. RESULTS: Narrowed disk height was not observed until 4 weeks after injury, and a significant change was observed at 9 weeks compared with the control L1-L2 level (P ˂ 0.05). MRI revealed disk degeneration beginning at 9 weeks and full degeneration at 15 weeks. Injured intervertebral disks had higher degeneration, seen using MRI, than uninjured control disks, Disk degeneration was confirmed in all injured levels by histologic examination. Cortical osteophyte formation was not found. CONCLUSIONS: Our percutaneous technique provides a suitable rabbit model of degenerative disk disease to test the safety and efficacy of treatments for disk degeneration, such as stem cell transplantation.
URI
http://www.sciencedirect.com/science/article/pii/S1878875015003666http://hdl.handle.net/20.500.11754/26968
ISSN
1878-8750; 1878-8769
DOI
10.1016/j.wneu.2015.03.066
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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