Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2017-04-21T02:42:38Z | - |
dc.date.available | 2017-04-21T02:42:38Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.citation | BIOCONJUGATE CHEMISTRY, v. 26, NO 8, Page. 1818-1829 | en_US |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.5b00357 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26868 | - |
dc.description.abstract | As an effective and safe strategy to overcome the limits of therapeutic nucleic acid or adenovirus (Ad) vectors for in vivo application, various technologies to modify the surface of vectors with nonimmunogenic/biocompatible polymers have been emerging in the field of gene therapy. However, the transfection efficacy of the polymer to transfer genetic materials is still relatively weak. To develop more advanced and effective polymers to deliver not only Ad vectors, but also nucleic acids, 6 biocompatible polymers were newly designed and synthesized to different sizes (2k, 3.4k, or 5k) of poly(ethylene) glycol (PEG) and different numbers of amine groups (2 or 5) based on methoxy poly(ethylene glycol)-b-poly{N-[N-(2-aminoethyl)-2-aminoethyl]-1,-glutamate (PNLG). We characterized size distribution and surface charge of 6 PNLGs after complexation with either nucleic acid or Ad. Among all 6 PNLGs, the 5 amine group PNLG showed the strongest efficacy in delivering nucleic acid as well as Ad vectors. Interestingly, cellular uptake results showed higher uptake ability in Ad complexed with 2 amine group PNLG than Ad/5 amine group PNLG, suggesting that the size of Ad/PNLGs is more essential than the surface charge for cellular uptake in polymers with charges greater than 30 mV. Moreover, the endosome escape ability of Ad/PNLGs increased depending on the number of amine groups, but decreased by PEG size. Cancer cell killing efficacy and immune response studies of oncolytic Ad/PNLGs showed 5 amine group PNLG to be a more effective and safe carrier for delivering Ad. Overall, these studies provide new insights into the functional mechanism of polymer-based approaches to either nucleic acid or Ad/nanocomplex. Furthermore, the identified ideal biocompatible PNLG polymer formulation (5 amine/2k PEG for nucleic acid, 5 amine/5k PEG for Ad) demonstrated high transduction efficiency as well as therapeutic value (efficacy and safety) and thus has strong potential for in vivo therapeutic use in the future. | en_US |
dc.description.sponsorship | This work was supported by grants from the National Research Foundation of Korea (2010 − 0029220,2013K1A1A2A02050188, 2013M3A9D3045879, to C.-O.Yun), the Basic Science Research Program through a National Research Foundation of Korea grant funded by the Korean Government (MEST) (20100027955, to D. S. Lee). The studies reported in this publication were supported by a grant from NIH CA177932. The author holds patent rights along with the University of Utah. Although a financial conflict of interest was identified for management based on the overall scope of the project and its potential benefit to the PI, the research findings included in this publication are not necessarily related to the interests of PI. The terms of this arrangement have been reviewed and approved by the University of Utah in accordance with its policy on objectivity in research. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | COMPLEXED ONCOLYTIC ADENOVIRUS | en_US |
dc.subject | GENE DELIVERY | en_US |
dc.subject | IN-VIVO | en_US |
dc.subject | BIOREDUCIBLE POLYMER | en_US |
dc.subject | NONVIRAL VECTORS | en_US |
dc.subject | TUMOR-GROWTH | en_US |
dc.subject | NEUTRALIZING ANTIBODIES | en_US |
dc.subject | THERAPEUTIC-EFFICACY | en_US |
dc.subject | CANCER-TREATMENT | en_US |
dc.subject | CELLS | en_US |
dc.title | Tuning Surface Charge and PEGylation of Biocompatible Polymers for Efficient Delivery of Nucleic Acid or Adenoviral Vector | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 26 | - |
dc.identifier.doi | 10.1021/acs.bioconjchem.5b00357 | - |
dc.relation.page | 1818-1829 | - |
dc.relation.journal | BIOCONJUGATE CHEMISTRY | - |
dc.contributor.googleauthor | Choi, Joung-Woo | - |
dc.contributor.googleauthor | KIm, Jaesung | - |
dc.contributor.googleauthor | Bui, Quang Nam | - |
dc.contributor.googleauthor | Li, Yi | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.contributor.googleauthor | Lee, Doo Sung | - |
dc.contributor.googleauthor | Kim, Sung Wan | - |
dc.relation.code | 2015000906 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
dc.identifier.researcherID | P-3698-2015 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-9466-4531 | - |
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