N-3 polyunsaturated fatty acids and 17 beta-estradiol injection induce antidepressant-like effects through regulation of serotonergic neurotransmission in ovariectomized rats

Title
N-3 polyunsaturated fatty acids and 17 beta-estradiol injection induce antidepressant-like effects through regulation of serotonergic neurotransmission in ovariectomized rats
Authors
박용순
Keywords
17 beta-Estradiol-3-benzoate; BDNF; Forced swimming test; Inflammatory cytokines; N-3 polyunsaturated fatty acids; Rats
Issue Date
2015-08
Publisher
ELSEVIER SCIENCE INC
Citation
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 26, NO 9, Page. 970-977
Abstract
Previous studies have suggested that estrogen and n-3 polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have antidepressant-like effects. The purpose of the present study was to determine the interaction between n-3 PUFAs and estrogen, and their neurotrophic mechanism in rats after the forced swimming test (FST). Rats were fed a modified American Institute of Nutrition 93G diet with 0%, 1% or 2% EPA+DHA relative to the total energy intake during 12 weeks. At 8 weeks, rats were ovariectomized and injected with either 17 beta-estradiol-3-benzoate (E-2) or corn oil during the last 3 weeks. Both n-3 PUFA supplementation and E-2 injection increased climbing and decreased immobility during the FST. Serum serotonin concentration was also increased by both n-3 PUFA and E-2. N-3 PUFA and E-2 decreased hippocampal expressions of interleukin (IL)-6 and tumor necrosis factor-alpha, and increased cAMP response element binding protein (CREB), phosphorylated CREB and brain-derived neurotrophic factor (BDNF). N-3 PUFA supplementation decreased hippocampal expression of IL-1 beta only in rats injected with E-2. Both n-3 PUPA supplementation and E-2 injection increased estrogen receptor (ER)-alpha in the hippocampus, but ER-beta was increased only by E-2 injection. Additionally, there was a significant interaction between n-3 PUFA supplementation and E-2 injection on the hippocampal expression of pCREB, suggesting membrane-mediated interaction of n-3 PUFAs and E-2. In conclusion, both n-3 PUFA and E-2 had antidepressant-like effects by regulating serotonergic neurotransmission through BDNF and inflammatory cytokines. (C) 2015 Elsevier Inc. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0955286315001047http://hdl.handle.net/20.500.11754/26791
ISSN
0955-2863; 1873-4847
DOI
http://dx.doi.org/10.1016/j.jnutbio.2015.04.005
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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