Toehold-mediated DNA displacement-based surface-enhanced Raman scattering DNA sensor utilizing an Au-Ag bimetallic nanodendrite substrate

Title
Toehold-mediated DNA displacement-based surface-enhanced Raman scattering DNA sensor utilizing an Au-Ag bimetallic nanodendrite substrate
Authors
정회일
Keywords
Toehold-mediated DNA strand displacement reaction; Surface-enhanced Raman scattering DNA sensor; Bimetallic nanodendrite; Signal-off sensor
Issue Date
2015-08
Publisher
ELSEVIER SCIENCE BV
Citation
ANALYTICA CHIMICA ACTA, v. 885, Page. 132-139
Abstract
A simple and sensitive surface enhanced Raman scattering (SERS)-based DNA sensor that utilizes the toehold-mediated DNA displacement reaction as a target-capturing scheme has been demonstrated. For a SERS substrate, Au-Ag bimetallic nanodendrites were electrochemically synthesized and used as a sensor platform. The incorporation of both Ag and Au was employed to simultaneously secure high sensitivity and stability of the substrate. An optimal composition of Ag and Au that satisfied these needs was determined. A double-strand composed of 'a probe DNA (pDNA)' complementary to 'a target DNA #tDNA#' and 'an indicator DNA tagged with a Raman reporter #iDNA#' was conjugated on the substrate. The conjugation made the reporter molecule close to the surface and induced generation of the Raman signal. The tDNA released the pre-hybridized iDNA from the pDNA via toehold-mediated displacement, and the displacement of the iDNA resulted in the decrease of Raman intensity. The variation of percent intensity change was sensitive and linear in the concentration range from 200 fM to 20 nM, and the achieved limit of detection #LOD# was 96.3 fM, superior to those reported in previous studies that adopted different signal taggings based on such as fluorescence and electrochemistry. #C# 2015 Elsevier B.V. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0003267015005310http://hdl.handle.net/20.500.11754/26788
ISSN
0003-2670; 1873-4324
DOI
http://dx.doi.org/10.1016/j.aca.2015.04.037
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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