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dc.contributor.author채영규-
dc.date.accessioned2017-03-31T04:45:26Z-
dc.date.available2017-03-31T04:45:26Z-
dc.date.issued2015-07-
dc.identifier.citationMOLECULAR BIOLOGY REPORTS, v. 42, no. 7, Page. 1233-1239en_US
dc.identifier.issn0301-4851-
dc.identifier.issn1573-4978-
dc.identifier.urihttp://link.springer.com/article/10.1007/s11033-015-3862-1-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/26483-
dc.description.abstractNeural stem cells (NSCs) can be differentiated into one of three cell lineages: neurons, astrocytes or, oligodendrocytes. Some neurotoxins have the ability to deregulate this dynamic process. NSC cell fate can be altered by ethanol as reported previously. Our aim was to investigate the alteration of genes by ethanol during NSC differentiation and to explore the molecular mechanism underlying this phenomenon. Here, mouse fetal forebrain derived NSCs were differentiated for 2 days with or without of ethanol (50 mM). We performed a comparative microarray analysis at day two using GeneChip(A (R)) Mouse Genome 430A 2.0 arrays. Microarray analysis showed that the expressions of 496 genes were altered by ethanol (56 and 440 were up- and down-regulated, respectively). Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed the association of the following altered genes in the Wnt signaling pathway: Wnt5a, Csnk2a1, Tcf7l2, Ccnd2, Nlk, Tbl1x, Tbl1xr1, Rac2 and Nfatc3. Quantitative real time PCR analysis also demonstrated the relative expression levels of these genes. As Wnt signaling is a player of brain development, ethanol-induced alterations may contribute to improper development of the brain. Our data could be a useful resource for elucidating the mechanism behind the ethanol neurotoxicity in developing brain.en_US
dc.description.sponsorshipThis research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2013R1A1A3011026 to K.H.J.) and (No. 2011-0030049 to Y.G.C.).en_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.subjectNeural stem cellsen_US
dc.subjectDifferentiationen_US
dc.subjectEthanolen_US
dc.subjectMicroarray analysisen_US
dc.subjectWnt signalingen_US
dc.titleTranscriptomic study of mouse embryonic neural stem cell differentiation under ethanol treatmenten_US
dc.typeArticleen_US
dc.relation.volume42-
dc.identifier.doi10.1007/s11033-015-3862-1-
dc.relation.page1233-1239-
dc.relation.journalMOLECULAR BIOLOGY REPORTS-
dc.contributor.googleauthorMandal, Chanchal-
dc.contributor.googleauthorPark, Ji Hyun-
dc.contributor.googleauthorChoi, Mi Ran-
dc.contributor.googleauthorKim, Sun Hwa-
dc.contributor.googleauthorBadejo, Abimbola Comfort-
dc.contributor.googleauthorChai, Jin Choul-
dc.contributor.googleauthorLee, Young Seek-
dc.contributor.googleauthorJung, Kyoung Hwa-
dc.contributor.googleauthorChai, Young Gyu-
dc.relation.code2015002343-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidygchai-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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