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Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation

Title
Deoxycholate-Based Glycosides (DCGs) for Membrane Protein Stabilisation
Author
채필석
Keywords
amphiphiles; deoxycholate; membrane proteins; molecular design; stabilization
Issue Date
2015-07
Publisher
WILEY-V C H VERLAG GMBH
Citation
CHEMBIOCHEM, v. 16, NO 10, Page. 1454-1459
Abstract
Detergents are an absolute requirement for studying the structure of membrane proteins. However, many conventional detergents fail to stabilise denaturation-sensitive membrane proteins, such as eukaryotic proteins and membrane protein complexes. New amphipathic agents with enhanced efficacy in stabilising membrane proteins will be helpful in overcoming the barriers to studying membrane protein structures. We have prepared a number of deoxycholate-based amphiphiles with carbohydrate head groups, designated deoxycholate-based glycosides (DCGs). These DCGs are the hydrophilic variants of previously reported deoxycholate-based N-oxides (DCAOs). Membrane proteins in these agents, particularly the branched diglucoside-bearing amphiphiles DCG-1 and DCG-2, displayed favourable behaviour compared to previously reported parent compounds (DCAOs) and conventional detergents (LDAO and DDM). Given their excellent properties, these agents should have significant potential for membrane protein studies.
URI
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201500151/abstract;jsessionid=32AFCFED05EFE6B753B683BF375499B6.f03t03http://hdl.handle.net/20.500.11754/26406
ISSN
1439-4227; 1439-7633
DOI
10.1002/cbic.201500151
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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