Reverse correlation with FXR expression of peripheral mononuclear cell and NPC1L1 expression of terminal ileum in non-alcoholic fatty liver disease.

Title
Reverse correlation with FXR expression of peripheral mononuclear cell and NPC1L1 expression of terminal ileum in non-alcoholic fatty liver disease.
Authors
전대원
Issue Date
2015-07
Publisher
WILEY-BLACKWELL
Citation
HEPATOLOGY, v. 62, NO Special SI, Page. 1289-1289(2218)
Abstract
Background: Niemann-Pick C1-Like 1 (NPC1L1) functions as sterol transporter to mediate intestinal cholesterol absorption. Although the role of Liver X receptor (LXR) and Farnesoid X receptor (FXR) in hepatic steatosis is well known, its correlation with LXR/FXR and intestinal cholesterol circulation has not been thoroughly studied. We investigated the associations among LXR, FXR and NPC1 L1 as well as its correlation with intestinal cholesterol circulation in patients with non-alcoholic fatty liver disease (NAFLD). Methods: We evaluated clinical characteristics from 25 NAFLD and 28 control patients. We conducted upper endoscopy, colonoscopy and non-enhanced CT. Fatty liver diagnosed with decreased liver/spleen HU. We calculated the expression level of LXR and FXR in peripheral mono-nuclear cell by qPCR. Immunohistochemical analysis (ABCA1, ABCG5/8, NPC1L1, SREBP, LXR, FXR, CD36) was carried out on tissue samples of duodenum and ileum from NAFLD patients. Results: The expression of LXR (p = 0.01) and FXR (p = 0.03) in mononuclear cells increased in NAFLD group compared to the control group. Expression of LXR, FXR, NPC1L1, ABCA1, ABCG5/8, and SREBP in ileum was decreased in fatty liver disease. The expression of LXR in mononuclear cell showed a negative correlation with the expression of LXR, FXR, ABCA1, ABCG8, SREBP, NPC1L1 in ileum. Expression of NPC1L1 (p = 0.15), FXR (p = 0.30) in ileum was decreased in patients with hypercholesterolemia. Expression of NPC1L1 (p = 0.03), LXR (duodenum) and LXR (ileum) was decreased as triglyceride increased. Conclusion: We can predict the expression of NPC1 L1 in terminal ileum by measuring the expression of LXR and FXR in peripheral mononuclear cell. Keywords: Liver X receptor, Farnesoid X receptor, NPC1L1, non-alcoholic fatty liver disease Disclosures: The following authors have nothing to disclose: Sang Bong Ahn, Dae Won Jun, Yong Kyun Cho, Eun Chul Jang, Seung Min Lee, Kiseok Jang
URI
http://onlinelibrary.wiley.com/doi/10.1002/hep.28240/fullhttp://hdl.handle.net/20.500.11754/26360
ISSN
0270-9139; 1527-3350
DOI
http://dx.doi.org/10.1002/hep.28240
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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