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Dexamethasone-Conjugated Polyamidoamine Dendrimer for Delivery of the Heme Oxygenase-1 Gene into the Ischemic Brain

Title
Dexamethasone-Conjugated Polyamidoamine Dendrimer for Delivery of the Heme Oxygenase-1 Gene into the Ischemic Brain
Author
이민형
Keywords
dexamethasone; gene therapy; heme oxygenase-1; polyamidoamine; stroke
Issue Date
2015-07
Publisher
WILEY-V C H VERLAG GMBH
Citation
MACROMOLECULAR BIOSCIENCE, v. 15, NO 7, Page. 1021-1028
Abstract
Heme oxygenase-1 (HO-1) has anti-apoptotic and anti-inflammatory effects. In this study, the HO-1 gene was delivered into the brain using dexamethasone-conjugated polyamidoamine generation 2 (PAMAMG2-Dexa) for the treatment of ischemic stroke. PAMAM G2-Dexa formed stable complexes with plasmid DNA (pDNA). The pDNA delivery efficiency of PAMAM G2-Dexa was higher than that of polyethylenimine (PEI25k, 25 kDa), dexamethasone-conjugated PEI (PEI-Dexa), and PAMAM G2 in Neuro2A cells. Therapeutic effect of PAMAM G2-Dexa/pHO-1 complexes was evaluated in a stroke animal model. PAMAM G2-Dexa delivered pHO-1 more efficiently into the ischemic brain than PEI25k and PEI-Dexa with higher therapeutic effect. Therefore, PAMAMG2-Dexa/pHO-1 complexes may be useful for ischemic stroke gene therapy.
URI
http://onlinelibrary.wiley.com/doi/10.1002/mabi.201500058/fullhttp://hdl.handle.net/20.500.11754/26166
ISSN
1616-5187; 1616-5195
DOI
10.1002/mabi.201500058
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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