Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경근 | - |
dc.date.accessioned | 2017-03-16T01:59:41Z | - |
dc.date.available | 2017-03-16T01:59:41Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | BIOMATERIALS, v. 65, Page. 163-174 | en_US |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.issn | 1878-5905 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0142961215005773 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26142 | - |
dc.description.abstract | Adenovirus (Ad) is a widely used vector for cancer gene therapy but its therapeutic efficacy is limited by low coxsackievirus and adenovirus receptor (CAR) expression in tumors and non-specifically targeted infection. Ad infectivity and specificity can be markedly improved by creating Ad-magnetic nanoparticles cluster complexes and directing their migration with an external magnetic field (MGF). We electrostatically complexed GFP-expressing, replication-incompetent Ad (dAd) with PEGylated and cross-linked iron oxide nanoparticles (PCION), generating dAd-PCION complexes. The dAd-PCION showed increased transduction efficiency, independent of CAR expression, in the absence or presence of an MGF. Cancer cell killing and intracellular oncolytic Ad (HmT)-PCION replication significantly increased with MGF exposure. Site-directed, magnetically-targeted delivery of the HmT-PCION elicited significantly greater therapeutic efficacy versus treatment with naked HmT or HmT-PCION without MGF in CAR-negative MCF7 tumors. Immunohistochemical tumor analysis showed increased oncolytic Ad replication in tumors following infection by HmT-PCION using an MGF. Whole-body bioluminescence imaging of tumor-bearing mice showed a 450-fold increased tumor-to-liver ratio for HmT-PCION with, versus without, MGF. These results demonstrate the feasibility and potential of external MGF-responsive PCION-coated oncolytic Ads as smart hybrid vectors for cancer gene therapy. (C) 2015 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by grants from the National Research Foundation of Korea (2010-0029220, 2013K1A1A2A02050188, and 2013M3A9D3045879 to Dr. C-O. Yun), the Korea Food and Drug Administration (KFDA-13172-306 to Dr. C-O. Yun), and the National Institutes of Health, USA (CA 177932 to Dr. C-O. Yun). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Cancer gene therapy | en_US |
dc.subject | Oncolytic adenovirus | en_US |
dc.subject | Magnetofection | en_US |
dc.subject | PEGylated and cross-linked iron oxide nanoparticles | en_US |
dc.subject | PCION | en_US |
dc.title | Using a magnetic field to redirect an oncolytic adenovirus complexed with iron oxide augments gene therapy efficacy | en_US |
dc.type | Article | en_US |
dc.relation.volume | 65 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2015.07.001 | - |
dc.relation.page | 163-174 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.contributor.googleauthor | Choi, Joung-Woo | - |
dc.contributor.googleauthor | Park, Ji Won | - |
dc.contributor.googleauthor | Na, Youjin | - |
dc.contributor.googleauthor | Jung, Soo-Jung | - |
dc.contributor.googleauthor | Hwang, June Kyu | - |
dc.contributor.googleauthor | Choi, Dongho | - |
dc.contributor.googleauthor | Lee, Kyeong Geun | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.relation.code | 2015001659 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hepafel | - |
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