Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination

Title
Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination
Authors
김영필
Keywords
TRANSFER BRET; PROTEIN INTERACTIONS; SINGLE-MOLECULE; CELLS; PURIFICATION; SERS
Issue Date
2015-07
Publisher
WILEY-V C H VERLAG GMBH
Citation
SMALL, v. 11, NO 28, Page. 3469-3475
Abstract
The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy-modified quantum dot (QD) acting as a donor-acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine-tagged Luc (Luc-His 6) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD-BRET reactions with metal ions (e.g., zinc ions), a geometry-controlled ZnO NR platform can facilitate the design of surface-initiated BRET sensors without being supplemented by copious metal ions: the geometry-controlled ZnO NR platform can therefore pave the way for nanostructure-based biosensors with enhanced analytical performance.
URI
http://onlinelibrary.wiley.com/doi/10.1002/smll.201403700/fullhttp://hdl.handle.net/20.500.11754/26085
ISSN
1613-6810; 1613-6829
DOI
http://dx.doi.org/10.1002/smll.201403700
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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