Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination
- Title
- Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination
- Author
- 김영필
- Keywords
- TRANSFER BRET; PROTEIN INTERACTIONS; SINGLE-MOLECULE; CELLS; PURIFICATION; SERS
- Issue Date
- 2015-07
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- SMALL, v. 11, NO 28, Page. 3469-3475
- Abstract
- The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy-modified quantum dot (QD) acting as a donor-acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine-tagged Luc (Luc-His 6) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD-BRET reactions with metal ions (e.g., zinc ions), a geometry-controlled ZnO NR platform can facilitate the design of surface-initiated BRET sensors without being supplemented by copious metal ions: the geometry-controlled ZnO NR platform can therefore pave the way for nanostructure-based biosensors with enhanced analytical performance.
- URI
- http://onlinelibrary.wiley.com/doi/10.1002/smll.201403700/fullhttp://hdl.handle.net/20.500.11754/26085
- ISSN
- 1613-6810; 1613-6829
- DOI
- 10.1002/smll.201403700
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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