Zn(II)-coordinated quantum dot-FRET nanosensors for the detection of protein kinase activity

Title
Zn(II)-coordinated quantum dot-FRET nanosensors for the detection of protein kinase activity
Authors
김태욱
Keywords
FRET; quantum dot; zinc; kinase; phosphorylation
Issue Date
2015-07
Publisher
MDPI AG
Citation
SENSORS, v. 15, NO 8, Page. 17977-17989
Abstract
We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that enabled the phosphorylated peptides to be strongly attached on the carboxyl groups of the QD surface via metal coordination, thus leading to a significant FRET efficiency. As a result, protein kinase activity in intermixed solution was efficiently detected by QD-FRET via Zn(II) coordination, especially when the peptide substrate was combined with affinity-based purification. We also found that mono- and di-phosphorylation in the peptide substrate could be discriminated by the Zn(II)-mediated QD-FRET. Our approach is expected to find applications for studying physiological function and signal transduction with respect to protein kinase activity.
URI
http://www.mdpi.com/1424-8220/15/8/17977/htmhttp://hdl.handle.net/20.500.11754/26077
ISSN
1424-8220
DOI
http://dx.doi.org/10.3390/s150817977
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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